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TFF2 Antibody (500-P312BT-25UG) in WB
Western Blot: To detect Human TFF-2 by Western Blot analysis TFF2 Polyclonal Antibody, Biotin (Product # 500-P312BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human TFF-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P312BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 12.0 kDa Recombinant Human TFF-2
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930339
Product Specific Information
AA Sequence of recombinant protein: AEKPSPCQCS RLSPHNRTNC GFPGITSDQC FDNGCCFDSS VTGVPWCFHP LPKQESDQCV MEVSDRRNCG YPGISPEECA SRKCCFSNFI FEVPWCFFPK SVEDCHY.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TFF-2. Anti-Human TFF-2-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human TFF-2 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human TFF-2 (500-P312) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TFF-2.
Western Blot: To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human TFF-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P312BT-1MG will be provided as 2 x 500 µg
Target Information
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion. SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SP; Spasmolysin; Spasmolytic polypeptide; spasmolytic protein 1; Trefoil factor 2
Gene Aliases: SML1; SP; TFF2
UniProt ID: (Human) Q03403
Entrez Gene ID: (Human) 7032
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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