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Invitrogen
TFF2 Polyclonal Antibody
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TFF2 Antibody (PA5-80112) in IHC (P)
Immunohistochemistry analysis of TFF2 on paraffin-embedded rat gastric tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with TFF2 polyclonal antibody (Product# PA5-80112) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Developmen... View More
Product Details
PA5-80112
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat TFF2 recombinant protein (Position: E24-Y129).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747227
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat gaster tissue, mouse gaster tissue. IHC: rat gaster tissue.
Target Information
Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing and protection of the gastrointestinal epithelia. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. SP (spasmolytic polypeptide), also designated Trefoil factor 2 (TFF2) precursor, is a trefoil protein that functions to inhibit gastrointestinal motility and gastric acid secretion. SP may also act as a structural component of the gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. A downregulation of SP expression is associated with primary gastric cancer, and a progressive loss of this protein is likely to be involved in the early stage of the multi-step gastric carcinogenesis pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: SP; Spasmolytic polypeptide; Trefoil factor 2; trefoil factor 2 (spasmolytic protein 1)
Gene Aliases: mSP; Sml1; SP; Tff2
UniProt ID: (Rat) Q09030
Entrez Gene ID: (Rat) 116592, (Mouse) 21785
positive regulation of cell proliferation
calcium-mediated signaling
regulation of cell migration
negative regulation of macrophage activation
negative regulation of inflammatory response
negative regulation of gastric acid secretion
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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