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Bioss
TGF alpha Polyclonal Antibody
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FIGURE: 1 / 5
TGF alpha Antibody (BS-0066R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TGF alpha Polyclonal Antibody, Unconjugated (bs-0066R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes an... View More
Product Details
BS-0066R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human TGF-alpha, amino acids 40-89.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protransforming growth factor alpha; TGF a; TGF α; TGF-alpha; TGFalpha; TGFα; Transforming growth factor; transforming growth factor, alpha; transforming growth factor-alpha; waved 1
Gene Aliases: RATTGFAA; TFGA; TGFA; TGFAA; wa-1; wa1
UniProt ID: (Human) P01135, (Mouse) P48030, (Rat) P01134
Entrez Gene ID: (Human) 7039, (Mouse) 21802, (Rat) 24827
activation of MAPK activity
ER to Golgi vesicle-mediated transport
cell proliferation
positive regulation of epidermal growth factor-activated receptor activity
positive regulation of mitotic nuclear division
COPII vesicle coating
positive regulation of epithelial cell proliferation
positive regulation of cell division
angiogenesis
epidermal growth factor receptor signaling pathway
positive regulation of cell proliferation
negative regulation of apoptotic process
negative regulation of cellular process
mammary gland alveolus development
hepatocyte proliferation
wound healing
response to drug
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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