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          • Primary Antibodies ›
          • TGF beta-1,2,3 Antibodies

          Invitrogen

          TGF beta-1,2,3 Monoclonal Antibody (1D11.16.8), Functional Grade, eBioscience™

          2 Published Figures
          9 References
          View all (11) TGF beta-1,2,3 antibodies

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          Cite TGF beta-1,2,3 Monoclonal Antibody (1D11.16.8), Functional Grade, eBioscience™

          • Published Figures (2)
          TGF beta-1,2,3 Antibody in Neutralization (Neu)
          Group 53 Created with Sketch.
          TGF beta-1,2,3 Antibody in Neutralization (Neu)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          TGF beta-1,2,3 Antibody (16-9243-85) in Neu

          Pathology oncology research : POR 2022 - FIGURE 2 Ets1 upregulation in glioblastoma endothelial cells depend on TGFbeta signaling. (A) RNA levels of Ets1 in bEND.3 cells treated with VEGFA, TNFalpha or TGFbeta (mean + SD of four independent experiments; ** p < 0.01; ANOVA with Tukey's multiple comparisons test). (B) RNA levels of Ets1 in bEND.3 cells treated with conditioned medium from CT-2A glioma cells with TGFbeta neutralizing antibody... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          TGF beta-1,2,3 Antibody in Neutralization (Neu)
          TGF beta-1,2,3 Antibody in Neutralization (Neu)

          Product Details

          16-9243-85

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-Dependent
          -

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          Assay-Dependent
          View 5 publications 5 publications

          Functional Assay (Functional)

          Assay-Dependent
          -

          Inhibition Assays (IA)

          -
          View 1 publication 1 publication

          T-Cell Activation (TCA)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Hamster, Human, Mouse, Non-human primate, Rhesus monkey, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1D11.16.8

          Conjugate

          Functional Grade Functional Grade Functional Grade

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2573124

          Product Specific Information

          Description: The monoclonal antibody 1D11.16.8 recognizes human, mouse, rat, hamster, canine and non-human primate Transforming Growth Factor (TGF) beta 1, 2 and 3. TGF beta is a pleiotropic cytokine that exists as five isoforms (TGF beta 1-5) that share 70-80% homology with each other. However, these isoforms are not homologous to TGF alpha. TGF beta 1 is highly conserved between human, murine, simian, bovine, porcine, and chicken proteins. TGF beta 1 is the most abundant isoform and is ubiquitously expressed by a variety of cell types, including platelets, macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. However, the other isoforms exhibit a more restricted expression pattern.

          TGF beta 1 is synthesized as a long precursor polypeptide, which is cleaved to yield the mature protein and the Latency Associated Peptide (LAP). LAP and mature TGF beta 1 remain non-covalently associated during secretion, forming homodimers known as the Small Latent Complex (SLC). Secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. The bioactivity of mature TGF beta 1 is dependent on its release from LAP by conformational changes and proteolytic processing. Its activities include inhibition of cell growth in epithelial cells, endothelial cells, fibroblasts, neurons, lymphoid cells, and other hematopoietic cell types. TGF beta 1 also inhibits the proliferation of T and NK cells, downregulates the activities of activated macrophages, and blocks the anti-tumor activity of IL-2 - bearing lymphokine-activated killer (LAK) cells. Recently, TGF beta 1 has been found to play a critical role in the development of regulatory T cells and act as a costimulatory factor for expression of Foxp3. Dendritic cells exposed to tumors have been reported to secrete TGF beta 1 and stimulate the differntiation of CD4+CD25+ Treg cell from peripheral CD4+CD25- progeny. TGF beta 1-induced regulatory T cells have been termed iTregs.

          Applications Reported: This 1D11.16.8 antibody has been reported for use in neutralization assays both in vivo and in vitro and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; tgf beta 1; tgf beta 3; tgf beta1; TGF-beta 1; TGF-beta-1; Transforming growth factor; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor beta1; transforming growth factor, beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease); transforming growth factor, beta-1; transforming growth factor-beta 1

          View more View less

          Gene Aliases: CED; DPD1; LAP; TGF-beta; TGF-beta1; TGFB; Tgfb-1; TGFB1; TGFbeta; TGFbeta1

          View more View less

          UniProt ID: (Human) P01137, (Rat) P17246, (Mouse) P04202

          View more View less

          Entrez Gene ID: (Human) 7040, (Rhesus monkey) 574135, (Dog) 403998, (Rat) 59086, (Chinese hamster) 100773359, (Mouse) 21803

          View more View less

          Function(s)
          glycoprotein binding antigen binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding protein homodimerization activity protein serine/threonine kinase activator activity protein heterodimerization activity protein N-terminus binding
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade regulation of cell growth ureteric bud development response to hypoxia morphogenesis of a branching structure cell activation epithelial to mesenchymal transition negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly SMAD protein import into nucleus negative regulation of neuroblast proliferation salivary gland morphogenesis endoderm development female pregnancy aging negative regulation of DNA replication cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to radiation response to wounding response to glucose defense response to fungus, incompatible interaction response to organic substance positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production response to organic cyclic compound cell growth regulation of striated muscle tissue development regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus negative regulation of cell-cell adhesion hyaluronan catabolic process T cell differentiation negative regulation of ossification negative regulation of cell growth lung development positive regulation of cell migration positive regulation of bone mineralization mammary gland development positive regulation of histone deacetylation organ regeneration positive regulation of protein complex assembly positive regulation of exit from mitosis lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone positive regulation of interleukin-17 production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation response to vitamin D response to laminar fluid shear stress positive regulation of histone acetylation positive regulation of protein dephosphorylation wound healing T cell activation regulation of cell proliferation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis response to drug myelination myeloid dendritic cell differentiation T cell homeostasis positive regulation of apoptotic process positive regulation of MAP kinase activity regulation of MAPK cascade protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling regulatory T cell differentiation cell-cell junction organization negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development inner ear development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion negative regulation of phagocytosis negative regulation of immune response negative regulation of T cell activation positive regulation of chemotaxis positive regulation of NF-kappaB transcription factor activity regulation of binding regulation of DNA binding positive regulation of smooth muscle cell differentiation negative regulation of release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling face morphogenesis frontal suture morphogenesis pathway-restricted SMAD protein phosphorylation positive regulation of SMAD protein import into nucleus SMAD protein signal transduction mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis negative regulation of gene silencing by miRNA regulation of cartilage development lens fiber cell differentiation response to cholesterol positive regulation of cell cycle arrest cellular response to growth factor stimulus cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of protein localization to nucleus positive regulation of NAD+ ADP-ribosyltransferase activity regulation of actin cytoskeleton reorganization regulation of miRNA metabolic process positive regulation of transcription regulatory region DNA binding connective tissue replacement involved in inflammatory response wound healing platelet degranulation Notch signaling pathway positive regulation of vascular endothelial growth factor production negative regulation of extracellular matrix disassembly macrophage derived foam cell differentiation oligodendrocyte development cell migration regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway regulation of interleukin-23 production negative regulation of interleukin-17 production regulation of apoptotic process positive regulation of vascular permeability positive regulation of regulatory T cell differentiation negative regulation of cell differentiation active induction of host immune response by virus positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of cell division regulation of blood vessel remodeling regulation of SMAD protein import into nucleus positive regulation of extracellular matrix assembly positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of receptor clustering regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of cardiac muscle cell differentiation skeletal system development CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment defense response skeletal muscle tissue development organ morphogenesis regulation of gene expression regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation growth cell development T-helper 17 cell lineage commitment
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