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          • Primary Antibodies ›
          • TGF beta-1,2,3 Antibodies

          Invitrogen

          TGF beta-1,2,3 Monoclonal Antibody (eBioTB2F), eBioscience™

          1 Published Figure
          1 Reference
          View all (11) TGF beta-1,2,3 antibodies

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          Cite TGF beta-1,2,3 Monoclonal Antibody (eBioTB2F), eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (1)
          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          TGF beta-1,2,3 Antibody (14-9943-81) in ELISA

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          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          TGF beta-1,2,3 Antibody in ELISA (ELISA)

          Product Details

          14-9943-81

          Applications
          Tested Dilution
          Publications

          ELISA (ELISA)

          6-10 µg/mL
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human

          Published species

          Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          eBioTB2F

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1271967

          Product Specific Information

          Description: The eBioTB2F antibody reacts with Transforming Growth Factor-beta (TGF-beta), a pleiotropic cytokine, exists in five isoforms, known as TGF-beta1-5. Homologies between isoforms range from 70-80% but no homology exists to TGF-alpha. TGF-beta1 is ubiquitous and the most abundant form found in lymphoid organs, while other isoforms are expressed in a more restricted distribution. The biologically active state of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-beta arise by proteolytic cleavage of longer precursors; the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-beta1 are identical and differ from mouse TGF-beta1 in a single amino acid. TGF-beta1 is produced in very high levels by platelets. Other cellular sources of TGF-beta1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-beta1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-beta1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-beta1 inhibits the proliferation of T cells and NK cells and down regulates the activities of activated macrophages. TGF-beta1 blocks the anti-tumor activity of IL-2 - bearing lymphokine-activated killer (LAK) cells.

          Recently, TGF-beta1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-beta1 and stimulate expansion of natural T reg cells. Moreover, TGF-beta1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-beta-induced regulatory T cells have been termed Ti-Treg.

          Applications Reported: This eBioTB2F antibody has been reported for use in ELISA.

          Applications Tested: The eBioTB2F antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human and mouse TGF-beta in combination with the detection eBio16TFB (Product # 13-9923-81) antibody for detection. A suitable range of concentrations of this antibody for ELISA capture is 6-10 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8000 pg/mL - 60 pg/mL should be included in each ELISA plate.

          This antibody recognizes the mature/active form of TGF beta 1 without association with Latency Associated Peptide (LAP). Most samples will require acid-treatment and neutralization to remove LAP from TGF beta 1 prior to evaluation in this assay. Samples should be tested in the assay immediately after acid treatment and neutralization. It is also possible that some serum and plasma samples may contain low levels of immunoreactive TGF beta 1 that has disassociated from LAP. Naturally occurring, free TGF beta 1 may be measurable by evaluating samples without acid treatment.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: latency-associated peptide; prepro-transforming growth factor beta-1; tgf beta 1; tgf beta 3; tgf beta1; TGF-beta-1; Transforming growth factor; Transforming growth factor beta-1 proprotein

          View more View less

          Gene Aliases: CED; DPD1; LAP; TGFB; TGFB1; TGFbeta

          View more View less

          UniProt ID: (Human) P01137

          View more View less

          Entrez Gene ID: (Human) 7040

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          Function(s)
          glycoprotein binding antigen binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding protein homodimerization activity protein serine/threonine kinase activator activity protein heterodimerization activity protein N-terminus binding
          Process(es)
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade ureteric bud development response to hypoxia epithelial to mesenchymal transition negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation connective tissue replacement involved in inflammatory response wound healing adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen platelet degranulation protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly SMAD protein import into nucleus Notch signaling pathway negative regulation of neuroblast proliferation salivary gland morphogenesis endoderm development female pregnancy aging negative regulation of DNA replication positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to radiation response to wounding response to glucose defense response to fungus, incompatible interaction positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of extracellular matrix disassembly positive regulation of epithelial to mesenchymal transition macrophage derived foam cell differentiation positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production oligodendrocyte development cell growth regulation of striated muscle tissue development cell migration regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus negative regulation of cell-cell adhesion hyaluronan catabolic process negative regulation of ossification negative regulation of cell growth regulation of cell migration positive regulation of cell migration positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone deacetylation organ regeneration positive regulation of protein complex assembly positive regulation of exit from mitosis lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation response to vitamin D response to laminar fluid shear stress positive regulation of histone acetylation positive regulation of protein dephosphorylation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis response to drug myelination regulation of apoptotic process myeloid dendritic cell differentiation T cell homeostasis positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of MAP kinase activity protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling regulatory T cell differentiation cell-cell junction organization positive regulation of regulatory T cell differentiation negative regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter active induction of host immune response by virus positive regulation of fibroblast proliferation positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development inner ear development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of phagocytosis positive regulation of chemotaxis positive regulation of NF-kappaB transcription factor activity regulation of binding regulation of DNA binding positive regulation of smooth muscle cell differentiation negative regulation of release of sequestered calcium ion into cytosol positive regulation of cell division positive regulation of protein kinase B signaling regulation of blood vessel remodeling face morphogenesis frontal suture morphogenesis pathway-restricted SMAD protein phosphorylation regulation of SMAD protein import into nucleus positive regulation of SMAD protein import into nucleus SMAD protein signal transduction mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis negative regulation of gene silencing by miRNA regulation of cartilage development lens fiber cell differentiation response to cholesterol positive regulation of cell cycle arrest cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of extracellular matrix assembly positive regulation of NAD+ ADP-ribosyltransferase activity positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of receptor clustering regulation of epithelial to mesenchymal transition involved in endocardial cushion formation regulation of actin cytoskeleton reorganization positive regulation of transcription regulatory region DNA binding positive regulation of cardiac muscle cell differentiation
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