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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TGF beta-2 Antibodies
          Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Invitrogen

          TGF beta-2 Recombinant Superclonal™ Antibody (12HCLC)

          Advanced Verification
          1 Published Figure
          1 Reference
          View all (17) TGF beta-2 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite TGF beta-2 Recombinant Superclonal™ Antibody (12HCLC)

          • Antibody Testing Data (3)
          • Published Figures (1)
          • Advanced Verification (1)
          TGF beta-2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          TGF beta-2 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          TGF beta-2 Antibody (710276) in WB

          Western blot analysis was performed on whole-cell extracts (30 µg lysate) of T98G (Lane 1), A549 (Lane 2), and NIH/3T3 (Lane 3). The blots were probed with Rabbit Anti-TGF beta-2 Recombinant Rabbit Superclonal™ Antibody (Product # 710276, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 47 kDa band corresponding to TGF beta-2 was observed across the cell lines tested. Known quantity of protein samples were el... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          TGF beta-2 Antibody in Western Blot (WB)
          TGF beta-2 Antibody in Western Blot (WB)
          TGF beta-2 Antibody in Flow Cytometry (Flow)
          TGF beta-2 Antibody in Western Blot (WB)
          TGF beta-2 Antibody

          Product Details

          710276

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Expression System

          Expi293

          Class

          Recombinant Superclonal

          Type

          Antibody

          Clone

          12HCLC

          Immunogen

          Recombinant protein corresponding to amino acids 331-442 of human Transforming Growth Factor Beta 2

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS

          Contains

          0.09% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Wet ice

          RRID

          AB_2532659

          Product Specific Information

          Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

          Target Information

          Transforming Growth Factor (TGF) betas mediate many cell to cell interactions that occur during embryonic development. Three TGF betas have been identified in mammals. TGF beta 1, TGF beta 2 and TGF beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: BSC-1 cell growth inhibitor; Cetermin; G-TSF; Glioblastoma-derived T-cell suppressor factor; polyergin; prepro-transforming growth factor beta-2; TGF-beta-2; TGFB; TGFβ2; Transforming growth factor; transforming growth factor beta-2; Transforming growth factor beta-2 proprotein; transforming growth factor, beta 2

          View more View less

          Gene Aliases: BB105277; LDS4; TGF-beta2; Tgfb-2; TGFB2

          View more View less

          UniProt ID: (Human) P61812, (Mouse) P27090

          View more View less

          Entrez Gene ID: (Human) 7042, (Mouse) 21808

          View more View less

          Function(s)
          beta-amyloid binding receptor signaling protein serine/threonine kinase activity receptor binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity type III transforming growth factor beta receptor binding protein homodimerization activity protein heterodimerization activity protein N-terminus binding
          Process(es)
          cell morphogenesis skeletal system development cartilage condensation blood vessel development eye development response to hypoxia epithelial to mesenchymal transition hair follicle development blood vessel remodeling heart morphogenesis outflow tract morphogenesis heart valve morphogenesis cardiac left ventricle morphogenesis cardiac right ventricle morphogenesis protein phosphorylation cell cycle arrest transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus axon guidance heart development cell death positive regulation of cell proliferation negative regulation of cell proliferation glial cell migration cardioblast differentiation positive regulation of gene expression positive regulation of epithelial cell migration negative regulation of alkaline phosphatase activity positive regulation of epithelial to mesenchymal transition positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production positive regulation of phosphatidylinositol 3-kinase signaling cell growth cell migration signal transduction by protein phosphorylation hemopoiesis extracellular matrix organization collagen fibril organization positive regulation of cell growth negative regulation of cell growth positive regulation of cell migration hair follicle morphogenesis activation of protein kinase activity response to progesterone positive regulation of stress-activated MAPK cascade regulation of transforming growth factor beta2 production positive regulation of cell adhesion mediated by integrin intracellular signal transduction growth wound healing regulation of cell proliferation dopamine biosynthetic process response to drug catagen regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process regulation of MAPK cascade positive regulation of neuron apoptotic process response to estrogen cell-cell junction organization negative regulation of keratinocyte differentiation positive regulation of integrin biosynthetic process positive regulation of cell cycle positive regulation of heart contraction somatic stem cell division neuron fate commitment neuron development embryonic neurocranium morphogenesis negative regulation of epithelial cell proliferation positive regulation of protein secretion negative regulation of immune response negative regulation of release of sequestered calcium ion into cytosol positive regulation of cell division positive regulation of catagen positive regulation of cardioblast differentiation cardiac muscle cell proliferation cardiac epithelial to mesenchymal transition pathway-restricted SMAD protein phosphorylation SMAD protein signal transduction ventricular septum morphogenesis negative regulation of cartilage development positive regulation of activation-induced cell death of T cells extrinsic apoptotic signaling pathway regulation of extracellular matrix organization positive regulation of extrinsic apoptotic signaling pathway in absence of ligand angiogenesis platelet degranulation outflow tract septum morphogenesis membranous septum morphogenesis atrioventricular valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis ventricular trabecula myocardium morphogenesis endocardial cushion fusion atrial septum primum morphogenesis cell-cell signaling salivary gland morphogenesis cell proliferation response to wounding embryo development negative regulation of gene expression neutrophil chemotaxis regulation of actin cytoskeleton organization ascending aorta morphogenesis odontogenesis uterine wall breakdown positive regulation of ossification negative regulation of Ras protein signal transduction embryonic digestive tract development generation of neurons positive regulation of immune response face morphogenesis atrial septum morphogenesis pharyngeal arch artery morphogenesis positive regulation of extracellular matrix disassembly positive regulation of protein localization to nucleus regulation of apoptotic process involved in outflow tract morphogenesis regulation of complement-dependent cytotoxicity positive regulation of GTP binding negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation
          It has to be done as per old AB suggested Products section.
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