Invitrogen

TGF beta-3 Monoclonal Antibody (20724)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-23812

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

8-25 µg/mL
-

Neutralization (Neu)

0.1-0.3 µg/mL
-
Product Specifications

Species Reactivity

Human, Many, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

20724

Immunogen

Sf 21-derived recombinant human TGF-beta 3 Ala301-Ser412 (Tyr340Phe)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609528

Product Specific Information

In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF- beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross-reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. This antibody neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

Transforming growth factor beta 3 (TGF beta 3) is the third member of the transforming growth factor family of cytokines, which also includes TGF beta 1 and beta 2. These cytokines are secreted in precursor form consisting of a bioactive C-terminal domain attached to an N-terminal domain known as latency associated protein (LAP). Cleavage of LAP results in the mature protein, which functions as a disulfide-linked homodimer. As with all members of the family, TGF beta 3 is highly conserved across species, with mouse and human TGF beta 3 demonstrating 100% sequence homology and cross-species activity.The members of this family can be expressed by most cell types and exert pleiotropic effects, which include the suppression of B- and T-cell effector activity, mediation of tissue healing, and suppression of tumor proliferation. The promotion of CD4+CD25+ T-cell expansion is a newly discovered function of the TGF beta cytokines, and indicates an important role in the protection against autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ16571; H-TGF-b-3; prepro-transforming growth factor beta-3; tgf beta 3; TGF-beta-3; Transforming growth factor; transforming growth factor beta-3; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 3

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Gene Aliases: ARVD; ARVD1; LDS5; RNHF; TGF-B3; TGF-beta3; Tgfb-3; TGFB3

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UniProt ID: (Human) P10600, (Rat) Q07258

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Entrez Gene ID: (Human) 7043, (Rat) 25717, (Mouse) 21809

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Function(s)
type II transforming growth factor beta receptor binding cytokine activity protein binding growth factor activity type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding identical protein binding protein heterodimerization activity transforming growth factor beta binding transforming growth factor beta receptor binding
Process(es)
activation of MAPK activity response to hypoxia in utero embryonic development platelet degranulation transforming growth factor beta receptor signaling pathway SMAD protein import into nucleus salivary gland morphogenesis female pregnancy aging negative regulation of DNA replication negative regulation of cell proliferation positive regulation of epithelial to mesenchymal transition positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production cell growth positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway mammary gland development response to progesterone positive regulation of collagen biosynthetic process response to laminar fluid shear stress wound healing regulation of cell proliferation odontogenesis uterine wall breakdown regulation of apoptotic process positive regulation of apoptotic process regulation of MAPK cascade negative regulation of neuron apoptotic process response to estrogen ossification involved in bone remodeling cell-cell junction organization positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter lung alveolus development digestive tract development embryonic neurocranium morphogenesis inner ear development positive regulation of protein secretion positive regulation of filopodium assembly positive regulation of stress fiber assembly positive regulation of cell division palate development face morphogenesis frontal suture morphogenesis positive regulation of SMAD protein import into nucleus SMAD protein signal transduction detection of hypoxia regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of occluding junction disassembly organ morphogenesis lung development regulation of epithelial cell differentiation regulation of endothelial cell differentiation cell development regulation of epithelial cell proliferation cellular response to growth factor stimulus embryo development growth
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