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THEM4 Antibody (PA5-110484) in WB
Western blot analysis of THEM4 in extracts of various cell lines. Samples were incubated with THEM4 Polyclonal antibody (Product # PA5-110484) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-110484
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 37-150 of human THEM4 (NP_444283.2).
Conjugate
Form
Liquid
Concentration
2.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855895
Product Specific Information
Immunogen sequence: SSEEVILKDC SVPNPSWNKD LRLLFDQFMK KCEDGSWKRL PSYKRTPTEW IQDFKTHFLD PKLMKEEQMS QAQLFTRSFD DGLGFEYVMF YNDIEKRMVC LFQGGPYLEG PPGF
Target Information
THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acyl-CoA thioesterase THEM4; Acyl-coenzyme A thioesterase THEM4; C-terminal modulator protein; Carboxyl-terminal modulator protein; Thioesterase superfamily member 4
Gene Aliases: 2700077M13Rik; 4921507I02Rik; CTMP; THEM4
UniProt ID: (Human) Q5T1C6, (Mouse) Q3UUI3
Entrez Gene ID: (Human) 117145, (Mouse) 75778
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