Proteintech

THOC5 Polyclonal Antibody

THOC5 Antibody in Immunocytochemistry (ICC/IF)

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THOC5 Antibody (14862-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (Product # 14862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

THOC5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

THOC5 Antibody in Immunoprecipitation (IP)

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Product Details

14862-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

THOC5 Fusion Protein Ag6664 (354-683 aa encoded by BC003615)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: CKDDSVLHL TFYYLMNLNI MTVKAKVTTA MELITPISAG DLLSPDSVLS CLYPGDHGKK TPNPANQYQF DKVGILTLSD YVLELGHPYL WVQKLGGLHF PKEQPQQTVI ADHSLSASHM ETTMKLLKTR VQSRLALHKQ FASLEHGIVP VTSDCQYLFP AKVVSRLVKW VTIAHEDYME LHFTKDIVDA GLAGDTNLYY MALIERGTAK LQAAVVLNPG YSSIPPIFQL CLNWKGEKTN SNDDNIRAME GEVNVCYKEL CGPWPSHQLL TNQLQRLCVL LDVYLETESH DDSVEGPKEF PQEKMCLRLF RGPSRMKPFK YNHPQGFFSH R (354-683 aa encoded by BC003615)

Target Information

Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and THOC4. UAP56 functions as a bridge between THOC4 and the THO complex. THOC5 in conjunction with THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. May be involved in the differentiation of granulocytes and adipocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The THO complex regulates pluripotency gene mRNA export and controls embryonic stem cell self-renewal and somatic cell reprogramming.

Cell stem cell

Wang L,Miao YL,Zheng X,Lackford B,Zhou B,Han L,Yao C,Ward JM,Burkholder A,Lipchina I,Fargo DC,Hochedlinger K,Shi Y,Williams CJ,Hu G

Thu Dec 05 00:00:00 UTC 2013

The THO complex regulates pluripotency gene mRNA export and controls embryonic stem cell self-renewal and somatic cell reprogramming.

Cell stem cell

Wang L,Miao YL,Zheng X,Lackford B,Zhou B,Han L,Yao C,Ward JM,Burkholder A,Lipchina I,Fargo DC,Hochedlinger K,Shi Y,Williams CJ,Hu G

Thu Dec 05 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: FMIP; Fms interacting protein; Fms-interacting protein; fSAP79; Functional spliceosome-associated protein 79; hTREX90; NF2/meningioma region protein pK1.3; Placental protein 39.2; PP39.2; serum/glucocorticoid regulated kinase 2; THO complex subunit 5; THO complex subunit 5 homolog

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Gene Aliases: 1700060C24Rik; A430085L24Rik; C22orf19; Fmip; fSAP79; KIAA0983; PK1.3; Sgk2; THOC5

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UniProt ID: (Human) Q13769, (Mouse) Q8BKT7, (Rat) Q68FX7

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Entrez Gene ID: (Human) 8563, (Mouse) 107829, (Rat) 360972

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Function(s)

mRNA binding protein binding RNA binding molecular_function

Process(es)

termination of RNA polymerase II transcription RNA export from nucleus mRNA export from nucleus RNA splicing monocyte differentiation mRNA 3'-end processing positive regulation of DNA-templated transcription, elongation viral mRNA export from host cell nucleus primitive hemopoiesis negative regulation of DNA damage checkpoint cell morphogenesis blastocyst development mRNA processing transport regulation of gene expression regulation of mRNA export from nucleus stem cell division cell differentiation negative regulation of macrophage differentiation mRNA transport regulation of stem cell division biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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THOC5 Polyclonal Antibody

THOC5 Antibody (14862-1-AP) in ICC/IF

Product Details

14862-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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