Invitrogen

THRA/THRB Monoclonal Antibody (C3)

THRA/THRB Antibody in Immunocytochemistry (ICC/IF)

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THRA/THRB Antibody (MA1-215) in ICC/IF

Immunofluorescent analysis of Thyroid Hormone Receptor in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Thyroid Hormone Receptor monoclonal antibody (Product # MA1-215) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Thyroid ... View More

THRA/THRB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

THRA/THRB Antibody in Western Blot, Immunoprecipitation (WB, IP)

THRA/THRB Antibody in Western Blot, ChIP Assay (WB, ChIP)

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Product Details

MA1-215

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:20

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 3 publications 3 publications

ChIP assay (ChIP)

View 5 publications 5 publications

Gel Shift (GS)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse, Non-human primate, Rat, Xenopus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Purified fragment of human TR beta-1 corresponding to residues 201-456.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325420

Product Specific Information

MA1-215 detects thyroid hormone receptor (TR) from human tissues. This antibody recognizes TR beta-1 and also cross-reacts with TR alpha-1.

MA1-215 has been successfully used in Western blot, IF, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 55 and a 52 kDa protein representing recombinant human TR beta-1 expressed in E. coli. MA1-215 immunoprecipitates 52 kDa and 55 kDa proteins, representing TR beta-1 and a 48 kDa protein representing TR alpha-1 expressed in E. coli.

The MA1-215 immunogen is a purified fragment of human TR beta-1 corresponding to amino acid residues 201-456. This antibody recognizes an epitope in the hormone binding domain of hTR beta-1, amino acid residues 235-414. This sequence is conserved in hTR alpha-1.

Target Information

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR), a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular histones, a new class of inhibitory molecules of CNS axonal regeneration.

Brain communications

Siddiq MM,Hannila SS,Zorina Y,Nikulina E,Rabinovich V,Hou J,Huq R,Richman EL,Tolentino RE,Hansen J,Velenosi A,Kwon BK,Tsirka SE,Maze I,Sebra R,Beaumont KG,Toro CA,Cardozo CP,Iyengar R,Filbin MT

MA1-215 was used in Protein Assay to identify extracellular histones as a new class of nerve regeneration-inhibiting molecules within the injured CNS.

Sat Jan 08 00:00:00 UTC 2022

Early life variations in temperature exposure affect the epigenetic regulation of the paraventricular nucleus in female rat pups.

Proceedings. Biological sciences

Lauby SC,McGowan PO

MA1-215 was used in Chromatin immunoprecipitation to suggest that repeated room temperature exposure may affect DNA methylation levels as a consequence of alterations in thyroid hormone receptor signalling.

Wed Oct 28 00:00:00 UTC 2020

The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome.

Human molecular genetics

Berner D,Hoja U,Zenkel M,Ross JJ,Uebe S,Paoli D,Frezzotti P,Rautenbach RM,Ziskind A,Williams SE,Carmichael TR,Ramsay M,Topouzis F,Chatzikyriakidou A,Lambropoulos A,Sundaresan P,Ayub H,Akhtar F,Qamar R,Zenteno JC,Cruz-Aguilar M,Astakhov YS,Dubina M,Wiggs J,Ozaki M,Kruse FE,Aung T,Reis A,Khor CC,Pasutto F,Schlötzer-Schrehardt U

MA1-215 was used in Electrophoretic mobility shift assay to determine the biological importance of pseudoexfoliation - associated common variants.

Thu Aug 01 00:00:00 UTC 2019

Reversing thyroid-hormone-mediated repression of a HSV-1 promoter via computationally guided mutagenesis.

Journal of cell science

Figliozzi RW,Chen F,Hsia SV

MA1-215 was used in ChIP assay to elucidate the impact on transcriptional repression of nucleotide sequence or orientation within thyroid hormone DNA-binding nuclear receptor binding sites.

Wed Nov 01 00:00:00 UTC 2017

MKK6 controls T3-mediated browning of white adipose tissue.

Nature communications

Matesanz N,Bernardo E,Acín-Pérez R,Manieri E,Pérez-Sieira S,Hernández-Cosido L,Montalvo-Romeral V,Mora A,Rodríguez E,Leiva-Vega L,Lechuga-Vieco AV,Ruiz-Cabello J,Torres JL,Crespo-Ruiz M,Centeno F,Álvarez CV,Marcos M,Enríquez JA,Nogueiras R,Sabio G

MA1-215 was used in Chromatin immunoprecipitation to demonstrate that MKK6 deletion in adipocytes increases white adipose tissue thermogenic capacity and this is a potential therapeutic strategy for obesity.

Wed Oct 11 00:00:00 UTC 2017

Thyroid Hormone Receptor-β (TRβ) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.

Endocrinology

Carr FE,Tai PW,Barnum MS,Gillis NE,Evans KG,Taber TH,White JH,Tomczak JA,Jaworski DM,Zaidi SK,Lian JB,Stein JL,Stein GS

MA1-215 was used in Gel Shift to study the relationship between TRB and Runx2 as regulatory factors in thyroid tumorigenesis.

Mon Aug 01 00:00:00 UTC 2016

Thyroid hormone-dependent epigenetic suppression of herpes simplex virus-1 gene expression and viral replication in differentiated neuroendocrine cells.

Journal of the neurological sciences

Figliozzi RW,Chen F,Balish M,Ajavon A,Hsia SV

MA1-215 was used in Western Blotting and ChIP to investigate whether triiodothyronine treatment of infected differentiated neuron-like cells reduces the ability of HSV-1 to produce viral progeny.

Sat Nov 15 00:00:00 UTC 2014

Thyroid hormone-dependent epigenetic suppression of herpes simplex virus-1 gene expression and viral replication in differentiated neuroendocrine cells.

Journal of the neurological sciences

Figliozzi RW,Chen F,Balish M,Ajavon A,Hsia SV

MA1-215 was used in Western Blotting and ChIP to investigate whether triiodothyronine treatment of infected differentiated neuron-like cells reduces the ability of HSV-1 to produce viral progeny.

Sat Nov 15 00:00:00 UTC 2014

The p160 coactivator PAS-B motif stabilizes nuclear receptor binding and contributes to isoform-specific regulation by thyroid hormone receptors.

The Journal of biological chemistry

Privalsky ML,Lee S,Hahm JB,Young BM,Fong RN,Chan IH

MA1-215 was used in western blot to investigate the relationship between p160 coactivators and thyroid hormone receptors.

Fri Jul 17 00:00:00 UTC 2009

Inhibition of apoptotic potency by ligand stimulated thyroid hormone receptors located in mitochondria.

Apoptosis : an international journal on programmed cell death

Saelim N,Holstein D,Chocron ES,Camacho P,Lechleiter JD

MA1-215 was used in western blot to investigate the role of thyroid hormone receptors in apoptosis

Mon Oct 01 00:00:00 UTC 2007

The rat thyroid hormone receptor (TR) Deltabeta3 displays cell-, TR isoform-, and thyroid hormone response element-specific actions.

Endocrinology

Harvey CB,Bassett JH,Maruvada P,Yen PM,Williams GR

MA1-215 was used in western blot to investigate the mechanism for rat thyroid hormone receptor Deltabeta3 actions.

Sun Apr 01 00:00:00 UTC 2007

Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone.

Proceedings of the National Academy of Sciences of the United States of America

Storey NM,Gentile S,Ullah H,Russo A,Muessel M,Erxleben C,Armstrong DL

MA1-215 was used in immunoprecipitation to investigate the molecular mechanism for the effects of thyroid hormone on human brain development.

Tue Mar 28 00:00:00 UTC 2006

Rapid signaling at the plasma membrane by a nuclear receptor for thyroid hormone.

Proceedings of the National Academy of Sciences of the United States of America

Storey NM,Gentile S,Ullah H,Russo A,Muessel M,Erxleben C,Armstrong DL

MA1-215 was used in immunoprecipitation to investigate the molecular mechanism for the effects of thyroid hormone on human brain development.

Tue Mar 28 00:00:00 UTC 2006

Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors.

The Journal of biological chemistry

Goodson ML,Jonas BA,Privalsky ML

MA1-215 was used in EMSA to investigate the alternative mRNA splicing of silencing mediator of retinoic acid and thyroid hormone receptors.

Fri Mar 04 00:00:00 UTC 2005

Alternative mRNA splicing of SMRT creates functional diversity by generating corepressor isoforms with different affinities for different nuclear receptors.

The Journal of biological chemistry

Goodson ML,Jonas BA,Privalsky ML

MA1-215 was used in EMSA to investigate the alternative mRNA splicing of silencing mediator of retinoic acid and thyroid hormone receptors.

Fri Mar 04 00:00:00 UTC 2005

Nontranscriptional modulation of intracellular Ca2+ signaling by ligand stimulated thyroid hormone receptor.

The Journal of cell biology

Saelim N,John LM,Wu J,Park JS,Bai Y,Camacho P,Lechleiter JD

MA1-215 was used in immunoprecipitation and western blot to study the regulation of IP3-mediated calcium signaling by thyroid hormone receptors.

Mon Dec 06 00:00:00 UTC 2004

Nontranscriptional modulation of intracellular Ca2+ signaling by ligand stimulated thyroid hormone receptor.

The Journal of cell biology

Saelim N,John LM,Wu J,Park JS,Bai Y,Camacho P,Lechleiter JD

MA1-215 was used in immunoprecipitation and western blot to study the regulation of IP3-mediated calcium signaling by thyroid hormone receptors.

Mon Dec 06 00:00:00 UTC 2004

Thyroid-hormone-dependent negative regulation of thyrotropin beta gene by thyroid hormone receptors: study with a new experimental system using CV1 cells.

The Biochemical journal

Nakano K,Matsushita A,Sasaki S,Misawa H,Nishiyama K,Kashiwabara Y,Nakamura H

MA1-215 was used in western blot to investigate the role of thyroid hormone receptors in regulation of thyrotropin beta gene

Mon Mar 01 00:00:00 UTC 2004

Multiple messenger ribonucleic acid variants regulate cell-specific expression of human thyroid hormone receptor beta1.

Molecular endocrinology (Baltimore, Md.)

Frankton S,Harvey CB,Gleason LM,Fadel A,Williams GR

MA1-215 was used in immunocytochemistry to demonstrate the function of human thyroid hormone receptor beta1 5-UTR mRNAs.

Thu Jul 01 00:00:00 UTC 2004

Thyroid hormone action in the absence of thyroid hormone receptor DNA-binding in vivo.

The Journal of clinical investigation

Shibusawa N,Hashimoto K,Nikrodhanond AA,Liberman MC,Applebury ML,Liao XH,Robbins JT,Refetoff S,Cohen RN,Wondisford FE

MA1-215 was used in western blot to investigate the possible role of DNA-binding in thyroid hormone's nuclear functions

Fri Aug 01 00:00:00 UTC 2003

Thyroid hormone receptor DNA binding is required for both positive and negative gene regulation.

The Journal of biological chemistry

Shibusawa N,Hollenberg AN,Wondisford FE

Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in ChIP assay

Fri Jan 10 00:00:00 UTC 2003

Thyroid hormone receptor DNA binding is required for both positive and negative gene regulation.

The Journal of biological chemistry

Shibusawa N,Hollenberg AN,Wondisford FE

Published figure using THRA/THRB monoclonal antibody (Product # MA1-215) in ChIP assay

Fri Jan 10 00:00:00 UTC 2003

Ligand-dependent degradation of retinoid X receptors does not require transcriptional activity or coactivator interactions.

Molecular and cellular biology

Osburn DL,Shao G,Seidel HM,Schulman IG

MA1-215 was used in western blot to study the agonist-stimulated RXR degradation

Wed Aug 01 00:00:00 UTC 2001

Cloning and characterization of two novel thyroid hormone receptor beta isoforms.

Molecular and cellular biology

Williams GR

MA1-215 was used in western blot to characterize two novel isoforms of thyroid hormone beta receptor.

Wed Nov 01 00:00:00 UTC 2000

Conformational changes of human beta 1 thyroid hormone receptor induced by binding of 3,3',5-triiodo-L-thyronine.

Biochemical and biophysical research communications

Bhat MK,Parkison C,McPhie P,Liang CM,Cheng SY

MA1-215 was used in immunoprecipitation to perform structural analysis of human thyroid hormone receptor beta 1

Tue Aug 31 00:00:00 UTC 1993

Bioinformatics

Protein Aliases: c-erbA-1; c-erbA-2; c-erbA-alpha; c-erbA-beta; EAR-7; ERBA-related 7; Nuclear receptor subfamily 1 group A member 1; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; THR; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor alpha; thyroid hormone receptor alpha 1; Thyroid hormone receptor beta; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); thyroid normone nuclear receptor alpha variant 1; TR; triiodothyronine receptor; V-erbA-related protein 7

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Gene Aliases: AR7; c-ERBA-1; C-ERBA-2; C-ERBA-BETA; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; ERBA2; GRTH; NR1A1; NR1A2; PRTH; THR1; THRA; THRA1; THRA2; THRB; THRB1; THRB2

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UniProt ID: (Human) P10827, (Human) P10828

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Entrez Gene ID: (Human) 7067, (Human) 7068

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Function(s)

transcription regulatory region sequence-specific DNA binding steroid receptor RNA activator RNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding steroid hormone receptor activity thyroid hormone receptor activity protein binding transcription factor binding zinc ion binding TBP-class protein binding protein domain specific binding chromatin DNA binding protein complex binding protein homodimerization activity transcription regulatory region DNA binding intronic transcription regulatory region DNA binding protein heterodimerization activity thyroid hormone binding DNA binding transcription corepressor activity enzyme binding sequence-specific DNA binding

Process(es)

cartilage condensation ossification kidney development liver development regulation of thyroid hormone mediated signaling pathway regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter brain development learning or memory regulation of heart contraction female courtship behavior response to cold hormone-mediated signaling pathway positive regulation of myotube differentiation negative regulation of RNA polymerase II transcriptional preinitiation complex assembly erythrocyte differentiation adrenal gland development intracellular receptor signaling pathway thyroid gland development response to nutrient levels regulation of myeloid cell apoptotic process response to drug cytoplasmic sequestering of transcription factor steroid hormone mediated signaling pathway negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of transcription, DNA-templated positive regulation of female receptivity positive regulation of transcription from RNA polymerase II promoter digestive tract development embryonic organ development regulation of lipid catabolic process Type I pneumocyte differentiation negative regulation of DNA-templated transcription, initiation negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated sensory perception of sound negative regulation of female receptivity organ morphogenesis

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THRA/THRB Monoclonal Antibody (C3)

THRA/THRB Antibody (MA1-215) in ICC/IF

Product Details

MA1-215

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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