Invitrogen

THRB Polyclonal Antibody

2 Published Figures

12 References

View all (8) THRB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Published Figures (2)

THRB Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

THRB Antibody (PA1-213A) in ICC/IF

Immunofluorescent analysis of Thyroid Hormone Receptor beta-1 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Thyroid Hormone Receptor beta-1 polyclonal antibody (Product # PA1-213A) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. C... View More

THRB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA1-213A

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

ChIP assay (ChIP)

View 3 publications 3 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C-I(62) F H L D H D D V N D Q S V S S A Q T F(81) of human TR beta-1, with an N-terminal added cysteine.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2203030

Product Specific Information

PA1-213A detects thyroid hormone receptor (TR) beta-1 from human and rat tissues. This antibody does not detect TR alpha-1 or TRv alpha-2.

PA1-213A has been successfully used in Western blot, immunohistochemistry (paraffin), IF and gel shift procedures. By Western blot, this antibody detects a ~55 kDa protein representing recombinant human TRv beta-1 expressed in E. coli.

PA1-213A immunizing peptide corresponds to residues 62-81 of human TR beta-1, with an N-terminal added cysteine. Alternative numbering of this protein has this peptide corresponding to residues 67-86 of human TR beta-1. This sequence is completely conserved between human and rat TR beta-1.

Target Information

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Triiodothyronine lowers the potential of colorectal cancer stem cells in vitro.

Oncology reports

Rostkowska O,Olejniczak-Kęder A,Spychalski P,Szaryńska M,Kobiela J

PA1-213A was used in Gel Shift, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to report that T3 exerts significant impact on CSCs of two CRC cell lines cultured in the form of colonospheres.

Sun Jan 01 00:00:00 UTC 2023

Thyroid hormone inhibits lung fibrosis in mice by improving epithelial mitochondrial function.

Nature medicine

Yu G,Tzouvelekis A,Wang R,Herazo-Maya JD,Ibarra GH,Srivastava A,de Castro JPW,DeIuliis G,Ahangari F,Woolard T,Aurelien N,Arrojo E Drigo R,Gan Y,Graham M,Liu X,Homer RJ,Scanlan TS,Mannam P,Lee PJ,Herzog EL,Bianco AC,Kaminski N

PA1-213A was used in Immunogold labeling to demonstrate that the antifibrotic properties of thyroid hormone are associated with protection of alveolar epithelial cells and restoration of mitochondrial function.

Mon Jan 01 00:00:00 UTC 2018

Thyroid hormone inhibits lung fibrosis in mice by improving epithelial mitochondrial function.

Nature medicine

Mon Jan 01 00:00:00 UTC 2018

Thyroid hormone receptor regulates most genes independently of fibroblast growth factor 21 in liver.

The Journal of endocrinology

Zhang A,Sieglaff DH,York JP,Suh JH,Ayers SD,Winnier GE,Kharitonenkov A,Pin C,Zhang P,Webb P,Xia X

PA1-213A was used in ChIP assay to elucidate the regulatory mechanisms of FGF21 expression by triiodothyronine and test if FGF21 is required for induction of hepatic triiodothyronine-responsive genes

Sun Mar 01 00:00:00 UTC 2015

Impaired hair growth and wound healing in mice lacking thyroid hormone receptors.

PloS one

Contreras-Jurado C,García-Serrano L,Martínez-Fernández M,Ruiz-Llorente L,Paramio JM,Aranda A

PA1-213A was used in immunohistochemistry - paraffin section to study the effect of thyroid hormone on hair growth and skin homeostasis

Fri Dec 04 00:00:00 UTC 2015

Barhl1 is directly regulated by thyroid hormone in the developing cerebellum of mice.

Biochemical and biophysical research communications

Dong H,Yauk CL,Wade MG

PA1-213A was used in ChIP assay to study the role of thyroid hormone in Barhl1 function in developing murine cerebellum

Fri Nov 11 00:00:00 UTC 2011

Thyroid hormone-regulated gene expression in juvenile mouse liver: identification of thyroid response elements using microarray profiling and in silico analyses.

BMC genomics

Paquette MA,Dong H,Gagné R,Williams A,Malowany M,Wade MG,Yauk CL

PA1-213A was used in ChIP assay to identify the thyroid response elements in the juvenile mouse liver

Thu Dec 29 00:00:00 UTC 2011

Aging impairs myocardial fatty acid and ketone oxidation and modifies cardiac functional and metabolic responses to insulin in mice.

American journal of physiology. Heart and circulatory physiology

Hyyti OM,Ledee D,Ning XH,Ge M,Portman MA

PA1-213A was used in western blot to investigate the effect of aging on myocardial fatty acid, ketone oxidation, and insulin sensitivity in mice

Wed Sep 01 00:00:00 UTC 2010

The dominant negative thyroid hormone receptor beta-mutant {Delta}337T alters PPAR{alpha} signaling in heart.

American journal of physiology. Endocrinology and metabolism

Buroker NE,Young ME,Wei C,Serikawa K,Ge M,Ning XH,Portman MA

PA1-213A was used in western blot to investigate the effect of thyroid hormone receptor beta-mutant for PPAR alpha signaling in heart

Thu Feb 01 00:00:00 UTC 2007

Multiple messenger ribonucleic acid variants regulate cell-specific expression of human thyroid hormone receptor beta1.

Molecular endocrinology (Baltimore, Md.)

Frankton S,Harvey CB,Gleason LM,Fadel A,Williams GR

PA1-213A was used in Immunocytochemistry to characterise the human thyroid hormone receptor beta1 gene.

Thu Jul 01 00:00:00 UTC 2004

TR expression and function in human bone marrow stromal and osteoblast-like cells.

The Journal of clinical endocrinology and metabolism

Siddiqi A,Parsons MP,Lewis JL,Monson JP,Williams GR,Burrin JM

PA1-213A was used in immunocytochemistry to investigate the functions of TR in human bone marrow stromal and osteoblast-like cells.

Fri Feb 01 00:00:00 UTC 2002

Expression of thyroid receptor isoforms in the human fetal central nervous system and the effects of intrauterine growth restriction.

Clinical endocrinology

Kilby MD,Gittoes N,McCabe C,Verhaeg J,Franklyn JA

PA1-213A was used in immunocytochemistry to investigate the effects of severe IUGR on the expression of TR isoforms in the human fetal central nervous system

Sun Oct 01 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA-BETA; MGC126109; MGC126110; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR; TR beta 1; TR-B1C-ERBA-2; TRbeta2Delta

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Gene Aliases: C-ERBA-2; C-ERBA-BETA; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3rec; THR1; THRB; THRB1; THRB2; TRbeta

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UniProt ID: (Human) P10828

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Entrez Gene ID: (Human) 7068, (Rat) 24831

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription corepressor activity thyroid hormone receptor activity protein binding zinc ion binding enzyme binding chromatin DNA binding sequence-specific DNA binding thyroid hormone binding double-stranded DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein homodimerization activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated transcription initiation from RNA polymerase II promoter sensory perception of sound negative regulation of female receptivity regulation of heart contraction female courtship behavior organ morphogenesis intracellular receptor signaling pathway steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter Type I pneumocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of cell proliferation regulation of lipid metabolic process positive regulation of chondrocyte differentiation positive regulation of ossification negative regulation of transcription, DNA-templated embryonic organ development regulation of cholesterol metabolic process regulation of triglyceride metabolic process

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THRB Polyclonal Antibody

THRB Antibody (PA1-213A) in ICC/IF

Product Details

PA1-213A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Immunomicroscopy (IM)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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