Invitrogen

THRB Monoclonal Antibody (J52)

11 Published Figures

32 References

View all (8) THRB antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (11)

THRB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 14

THRB Antibody (MA1-216) in IHC (P)

Immunohistochemistry analysis of Thyroid Hormone Receptor beta-1 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were bloc... View More

THRB Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP)

THRB Antibody in Western Blot, Gel Shift (WB, GS)

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Product Details

MA1-216

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

ChIP assay (ChIP)

View 5 publications 5 publications

Gel Shift (GS)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

J52

Immunogen

Purified human TR beta -1 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2287303

Product Specific Information

MA1-216 detects thyroid hormone receptor (TR) beta-1 in human, mouse, rat, and transformed bacterial cells. This antibody is specific for the TR beta-1 isoform.

MA1-216 has been successfully used in Western blot, immunocytochemistry, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects a 52 and a 55 kDa protein representing recombinant TR expressed in E. coli. The two different sizes represent two different translational starting points. Immunocytochemical staining of TR beta-1 in intact GH3 cells with MA1-216 results in nuclear staining. When levels of expression of the transfected receptor are high, cytoplasmic staining is seen along with nuclear staining. Immunoprecipitation and gel super shift experiments show that MA1-216 reacts with the unliganded, liganded, and DNA binding forms of the receptor.

The MA1-216 immunogen is purified human TR beta-1 overexpressed in E. coli. This antibody recognizes an epitope in the A/B domain of human TR beta-1, amino acid residues 1-101.

Target Information

Thyroid hormone receptors (TR), also known as c-erbA-2, are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences called hormone response elements following activation by the appropriate hormone. Thyroid hormones, through their interaction with TR, effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins. There are two forms of TR; TR alpha (on chromosome 17) and TR beta (on chromosome 3). Each of these isoforms also has two isoforms; TR alpha-1 and TRv alpha-2, and TR beta-1 and TR beta-2 respectively. TR alpha-1 and 2 are identical through amino acid 370 where their sequences diverge. TRv alpha-2, which does not bind T3 and is a strong negative regulator of the functional TR, is 80 amino acids longer than TR alpha-1. The corresponding gene for the thyroid hormone receptor beta-1 is NR1A2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low striatal T3 is implicated in inattention and memory impairment in an ADHD mouse model overexpressing thyroid hormone-responsive protein.

Communications biology

Custodio RJP,Kim M,Sayson LV,Lee HJ,Ortiz DM,Kim BN,Kim HJ,Cheong JH

Published figure using THRB monoclonal antibody (Product # MA1-216) in Western Blot

Mon Sep 20 00:00:00 UTC 2021

Changes in Thyroid Hormone Signaling Mediate Cardiac Dysfunction in the Tg197 Mouse Model of Arthritis: Potential Therapeutic Implications.

Journal of clinical medicine

Ntari L,Mantzouratou P,Katsaouni A,Pantos C,Kollias G,Mourouzis I

MA1-216 was used in Immunohistochemistry and Western Blotting-immunofluorescence to investigate possible causes of cardiac dysfunction related to thyroid hormone (TH) signaling in a Rheumatoid Arthritis mouse model.

Thu Nov 25 00:00:00 UTC 2021

Changes in Thyroid Hormone Signaling Mediate Cardiac Dysfunction in the Tg197 Mouse Model of Arthritis: Potential Therapeutic Implications.

Journal of clinical medicine

Ntari L,Mantzouratou P,Katsaouni A,Pantos C,Kollias G,Mourouzis I

Thu Nov 25 00:00:00 UTC 2021

Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells.

Oncology letters

Peng X,Zhang Y,Sun Y,Wang L,Song W,Li Q,Zhao R

MA1-216 was used in Western Blotting to examine the effects of overexpression of thyroid hormone receptor Β1 in human breast cancer cells.

Sun Jul 01 00:00:00 UTC 2018

Thyroid Hormone Receptor-β (TRβ) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.

Endocrinology

Carr FE,Tai PW,Barnum MS,Gillis NE,Evans KG,Taber TH,White JH,Tomczak JA,Jaworski DM,Zaidi SK,Lian JB,Stein JL,Stein GS

MA1-216 was used in Western Blotting to study the relationship between TRB and Runx2 as regulatory factors in thyroid tumorigenesis.

Mon Aug 01 00:00:00 UTC 2016

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

MA1-216 was used in western blot to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

MA1-216 was used in western blot to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

MA1-216 was used in western blot to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation.

The Journal of biological chemistry

Liu YY,Ayers S,Milanesi A,Teng X,Rabi S,Akiba Y,Brent GA

MA1-216 was used in western blot to investigate thyroid hormone receptor in adipogenesis

Fri Mar 20 00:00:00 UTC 2015

Changes in thyroid hormone receptors after permanent cerebral ischemia in male rats.

Journal of molecular neuroscience : MN

Lourbopoulos A,Mourouzis I,Karapanayiotides T,Nousiopoulou E,Chatzigeorgiou S,Mavridis T,Kokkinakis I,Touloumi O,Irinopoulou T,Chouliaras K,Pantos C,Karacostas D,Grigoriadis N

MA1-216 was used in western blot to study the effect of permanent cerebral ischemia on the expression of thyroid hormone receptors in a male rat model

Mon Sep 01 00:00:00 UTC 2014

Genome-wide binding patterns of thyroid hormone receptor beta.

PloS one

Ayers S,Switnicki MP,Angajala A,Lammel J,Arumanayagam AS,Webb P

MA1-216 was used in ChIP assay and EMSA to perform a genome-wide analysis of thyroid hormone receptor-beta binding using BioChIP in HepG2 cells

Tue Jan 13 00:00:00 UTC 2015

Genome-wide binding patterns of thyroid hormone receptor beta.

PloS one

Ayers S,Switnicki MP,Angajala A,Lammel J,Arumanayagam AS,Webb P

MA1-216 was used in ChIP assay and EMSA to perform a genome-wide analysis of thyroid hormone receptor-beta binding using BioChIP in HepG2 cells

Tue Jan 13 00:00:00 UTC 2015

Genome-wide binding patterns of thyroid hormone receptor beta.

PloS one

Ayers S,Switnicki MP,Angajala A,Lammel J,Arumanayagam AS,Webb P

MA1-216 was used in ChIP assay and EMSA to perform a genome-wide analysis of thyroid hormone receptor-beta binding using BioChIP in HepG2 cells

Tue Jan 13 00:00:00 UTC 2015

Inhibition of thyroid hormone receptor α1 impairs post-ischemic cardiac performance after myocardial infarction in mice.

Molecular and cellular biochemistry

Mourouzis I,Kostakou E,Galanopoulos G,Mantzouratou P,Pantos C

MA1-216 was used in western blot to study the deleterious effect of pharmacological inhibition of thyroid hormone receptor alpha1 on post-ischemic cardiac function in a murine model of myocardial infarction

Mon Jul 01 00:00:00 UTC 2013

Thyroid hormone signalling is altered in response to physical training in patients with end-stage heart failure and mechanical assist devices: potential physiological consequences?

Interactive cardiovascular and thoracic surgery

Adamopoulos S,Gouziouta A,Mantzouratou P,Laoutaris ID,Dritsas A,Cokkinos DV,Mourouzis I,Sfyrakis P,Iervasi G,Pantos C

MA1-216 was used in western blot to study the enhanced thyroid signalling observed in end-stage heart failure patients with ventricular assist devices following exercise training

Tue Oct 01 00:00:00 UTC 2013

Nuclear corepressors mediate the repression of phospholipase A2 group IIa gene transcription by thyroid hormone.

The Journal of biological chemistry

Sharma P,Thakran S,Deng X,Elam MB,Park EA

MA1-216 was used in ChIP assay to study the mechanism by which thyroid hormone T3 inhibits the expression of secretory phospholipase A2 group IIa

Fri Jun 07 00:00:00 UTC 2013

Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by CCAAT/enhancer-binding protein beta (C/EBPbeta).

The Journal of biological chemistry

Attia RR,Sharma P,Janssen RC,Friedman JE,Deng X,Lee JS,Elam MB,Cook GA,Park EA

MA1-216 was used in ChIP assay to investigate the importance of CCAAT/enhancer-binding protein beta in the regulation of pyruvate dehydrogenase kinase 4 expression

Fri Jul 08 00:00:00 UTC 2011

Regulation of pyruvate dehydrogenase kinase 4 (PDK4) by CCAAT/enhancer-binding protein beta (C/EBPbeta).

The Journal of biological chemistry

Attia RR,Sharma P,Janssen RC,Friedman JE,Deng X,Lee JS,Elam MB,Cook GA,Park EA

MA1-216 was used in ChIP assay to investigate the importance of CCAAT/enhancer-binding protein beta in the regulation of pyruvate dehydrogenase kinase 4 expression

Fri Jul 08 00:00:00 UTC 2011

Cell-type-dependent thyroid hormone effects on glioma tumor cell lines.

Journal of thyroid research

Liappas A,Mourouzis I,Zisakis A,Economou K,Lea RW,Pantos C

MA1-216 was used in western blot to study the effect of thyroid hormone on glioma cells

Thu Aug 23 00:00:00 UTC 2012

Up-regulation of type 2 iodothyronine deiodinase in dilated cardiomyopathy.

Cardiovascular research

Wang YY,Morimoto S,Du CK,Lu QW,Zhan DY,Tsutsumi T,Ide T,Miwa Y,Takahashi-Yanaga F,Sasaguri T

MA1-216 was used in western blot to investigate the possible mechanism of the pathogenesis of DCM caused by hyperthyroidism using a konck-in mouse model

Wed Sep 01 00:00:00 UTC 2010

Thyroid hormone can favorably remodel the diabetic myocardium after acute myocardial infarction.

Molecular and cellular biochemistry

Kalofoutis C,Mourouzis I,Galanopoulos G,Dimopoulos A,Perimenis P,Spanou D,Cokkinos DV,Singh J,Pantos C

MA1-216 was used in western blot to investigate the effect of thyroid hormone on remodelling the diabetic myocardium

Wed Dec 01 00:00:00 UTC 2010

Thyroid hormone receptor alpha1 downregulation in postischemic heart failure progression: the potential role of tissue hypothyroidism.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme

Pantos C,Mourouzis I,Galanopoulos G,Gavra M,Perimenis P,Spanou D,Cokkinos DV

MA1-216 was used in western blot to investigate the downregulation of thyroid hormone receptor alpha1 in postischemic heart failure progression

Wed Sep 01 00:00:00 UTC 2010

TNF-alpha administration in neonatal cardiomyocytes is associated with differential expression of thyroid hormone receptors: a response prevented by T3.

Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme

Pantos C,Xinaris C,Mourouzis I,Kokkinos AD,Cokkinos DV

MA1-216 was used in western blot to investigate the effect of tumor necrosis factor alpha administration on thyroid hormone receptors in neonatal cardiomyocytes

Wed Oct 01 00:00:00 UTC 2008

Triiodothyronine accelerates differentiation of rat liver progenitor cells into hepatocytes.

Histochemistry and cell biology

László V,Dezso K,Baghy K,Papp V,Kovalszky I,Sáfrány G,Thorgeirsson SS,Nagy P,Paku S

MA1-216 was used in immunohistochemistry to investigate the effect of triiodothyronine on rat liver hepatocyte development

Sat Nov 01 00:00:00 UTC 2008

Time-dependent changes in the expression of thyroid hormone receptor alpha 1 in the myocardium after acute myocardial infarction: possible implications in cardiac remodelling.

European journal of endocrinology

Pantos C,Mourouzis I,Xinaris C,Kokkinos AD,Markakis K,Dimopoulos A,Panagiotou M,Saranteas T,Kostopanagiotou G,Cokkinos DV

MA1-216 was used in western blot to investigate the expression of thyroid hormone receptor alpha 1 in the myocardium after acute myocardial infarction

Sun Apr 01 00:00:00 UTC 2007

Thyroid hormone attenuates cardiac remodeling and improves hemodynamics early after acute myocardial infarction in rats.

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Pantos C,Mourouzis I,Markakis K,Dimopoulos A,Xinaris C,Kokkinos AD,Panagiotou M,Cokkinos DV

MA1-216 was used in western blot to investigate the role of thyroid hormone on cardiac remodeling and myocardial performance in rat

Wed Aug 01 00:00:00 UTC 2007

Thyroid hormone receptors alpha1 and beta1 are downregulated in the post-infarcted rat heart: consequences on the response to ischaemia-reperfusion.

Basic research in cardiology

Pantos C,Mourouzis I,Saranteas T,Paizis I,Xinaris C,Malliopoulou V,Cokkinos DV

MA1-216 was used in western blot to investigate the function of thyroid hormone signaling in the response of heart to ischaemia-reperfusion after myocardial infarction

Thu Sep 01 00:00:00 UTC 2005

p62, A TFIIH subunit, directly interacts with thyroid hormone receptor and enhances T3-mediated transcription.

Molecular endocrinology (Baltimore, Md.)

Liu Y,Ando S,Xia X,Yao R,Kim M,Fondell J,Yen PM

MA1-216 was used in chromatin immunoprecipitation to study the interaction between the TFIIH subunit p62 and thyroid hormone receptor and its effect on gene transcription.

Fri Apr 01 00:00:00 UTC 2005

Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity.

Molecular biology of the cell

Maruvada P,Dmitrieva NI,East-Palmer J,Yen PM

MA1-216 was used in western blot to demonstrate the cell cycle-dependent expression of thyroid hormone receptor-beta.

Thu Apr 01 00:00:00 UTC 2004

Thyroid status affects number and localization of thyroid hormone receptor expressing mast cells in bone marrow.

Bone

Siebler T,Robson H,Bromley M,Stevens DA,Shalet SM,Williams GR

MA1-216 was used in immunohistochemistry to investigate the association between thyroid status and thyroid hormone receptor expressing mast cells

Tue Jan 01 00:00:00 UTC 2002

Increased expression of thyroid hormone receptor isoforms in end-stage human congestive heart failure.

The Journal of clinical endocrinology and metabolism

d'Amati G,di Gioia CR,Mentuccia D,Pistilli D,Proietti-Pannunzi L,Miraldi F,Gallo P,Celi FS

MA1-216 was used in western blot to investigate the expression status of thyroid hormone receptor isoforms in end-stage human myocardial failure .

Tue May 01 00:00:00 UTC 2001

Overexpression of thyroid hormone receptor beta1 is associated with thyrotropin receptor gene expression and proliferation in a human thyroid carcinoma cell line.

The Journal of endocrinology

Chen ST,Shieh HY,Lin JD,Chang KS,Lin KH

MA1-216 was used in western blot to investigate the association of thyroid hormone receptor beta1 and thyrotropin receptor

Mon May 01 00:00:00 UTC 2000

Thyroid hormone affects Schwann cell and oligodendrocyte gene expression at the glial transition zone of the VIIIth nerve prior to cochlea function.

Development (Cambridge, England)

Knipper M,Bandtlow C,Gestwa L,Köpschall I,Rohbock K,Wiechers B,Zenner HP,Zimmermann U

MA1-216 was used in western blot to examine the effects of thyroid hormone on the expression of oligodendrocyte gene at the glial transition zone of the VIIIth nerve

Tue Sep 01 00:00:00 UTC 1998

Variable transcriptional activity and ligand binding of mutant beta 1 3,5,3'-triiodothyronine receptors from four families with generalized resistance to thyroid hormone.

Molecular endocrinology (Baltimore, Md.)

Meier CA,Dickstein BM,Ashizawa K,McClaskey JH,Muchmore P,Ransom SC,Menke JB,Hao EH,Usala SJ,Bercu BB

MA1-216 was used in immunocytochemistry to characterize TR beta 1 mutants

Sat Feb 01 00:00:00 UTC 1992

Bioinformatics

Protein Aliases: c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA-BETA; MGC126109; MGC126110; Nuclear receptor subfamily 1 group A member 2; oncogene ERBA2; thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta; thyroid hormone receptor beta 3; thyroid hormone receptor beta2delta; thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2); thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian); TR; TR beta; TR beta 1; TR-B1C-ERBA-2; TRbeta2Delta

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Gene Aliases: C-ERBA-2; C-ERBA-BETA; c-erbAbeta; ERBA2; GRTH; NR1A2; PRTH; RATT3REC; T3R[b]; T3Rbeta; T3rec; THR1; THRB; THRB1; THRB2; TRbeta

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UniProt ID: (Human) P10828, (Mouse) P37242

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Entrez Gene ID: (Human) 7068, (Mouse) 21834, (Rat) 24831

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity transcription corepressor activity thyroid hormone receptor activity protein binding zinc ion binding enzyme binding chromatin DNA binding sequence-specific DNA binding thyroid hormone binding double-stranded DNA binding protein homodimerization activity metal ion binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter sensory perception of sound negative regulation of female receptivity regulation of heart contraction female courtship behavior positive regulation of cell proliferation organ morphogenesis regulation of lipid metabolic process intracellular receptor signaling pathway positive regulation of chondrocyte differentiation steroid hormone mediated signaling pathway positive regulation of ossification negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter Type I pneumocyte differentiation regulation of cholesterol metabolic process regulation of triglyceride metabolic process transcription initiation from RNA polymerase II promoter embryonic organ development

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THRB Monoclonal Antibody (J52)

THRB Antibody (MA1-216) in IHC (P)

Product Details

MA1-216

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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