Proteintech

TID1 Polyclonal Antibody

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View all (9) TID1 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

11088-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunoprecipitation (IP)

0.5-4.0 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TID1 Fusion Protein Ag1558 (181-480 aa encoded by BC011855)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: DPEELFRKI FGEFSSSSFG DFQTVFDQPQ EYFMELTFNQ AAKGVNKEFT VNIMDTCERC NGKGNEPGTK VQHCHYCGGS GMETINTGPF VMRSTCRRCG GRGSIIISPC VVCRGAGQAK QKKRVMIPVP AGVEDGQTVR MPVGKREIFI TFRVQKSPVF RRDGADIHSD LFISIAQALL GGTARAQGLY ETINVTIPPG TQTDQKIRMG GKGIPRINSY GYGDHYIHIK IRVPKRLTSR QQSLILSYAE DETDVEGTVN GVTLTSSGGS TMDSSAGSKA RREAGEDEEG FLSKLKKMFT S (181-480 aa encoded by BC011855)

Target Information

TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid and encodes two mitochondrial matrix localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are the conserved members of the DnaJ family of proteins which act as cochaperones for mitochondrial Hsp70. They contain a conserved tetrahedrical J domain which binds to Hsp70 chaperones and activates their ATPase activity. Expression of hTid-1L increases apoptosis induced by DNA damaging agents as mitomycin-C and TNF-a. A J-domain mutant of hTid-1L can dominantly suppress apoptosis and in sharp contrast the J-domain mutant of hTid-1S increases apoptosis. Expression of hTid-1S and hTid-1L affects cytochrome c release from the mitochondria and caspase 3 activation, while activation of caspase 8 is unaffected. It is strongly suggested that these two splice variants exert their anti- and pro- apoptotic effects through discrete substrates and activities. Hence the relative abundance of these proteins or their substrates may allow the mitochondria to dampen or enhance the apoptotic signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DnaJ (Hsp40) homolog, subfamily A, member 3; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; hTid-1; mTid-1; Tumorous imaginal discs protein Tid56 homolog

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Gene Aliases: 1200003J13Rik; 1810053A11Rik; C81173; DNAJA3; HCA57; hTID-1; Tid-1; TID1; Tid1l

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UniProt ID: (Human) Q96EY1, (Mouse) Q99M87

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Entrez Gene ID: (Human) 9093, (Mouse) 83945, (Rat) 360481

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Function(s)
protein binding ATP binding Hsp70 protein binding GTPase regulator activity receptor tyrosine kinase binding metal ion binding unfolded protein binding interferon-gamma receptor binding transcription factor binding protein kinase binding heat shock protein binding NF-kappaB binding chaperone binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter mitochondrial DNA replication protein folding negative regulation of protein kinase activity apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process activation-induced cell death of T cells mitochondrion organization small GTPase mediated signal transduction neuromuscular junction development cell aging negative regulation of cell proliferation response to heat embryo development positive regulation of protein ubiquitination negative regulation of NF-kappaB transcription factor activity T cell differentiation in thymus response to interferon-gamma protein refolding positive regulation of T cell proliferation regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of programmed cell death negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of catalytic activity protein stabilization negative regulation of interferon-gamma-mediated signaling pathway skeletal muscle acetylcholine-gated channel clustering
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