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Invitrogen
TIF1 gamma Polyclonal Antibody
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FIGURE: 1 / 10
TIF1 gamma Antibody (PA5-80187) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of TIF1 gamma in A431 cells using TIF1 gamma Polyclonal Antibody (Product # PA5-80187). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 ... View More
Product Details
PA5-80187
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TIF1 gamma recombinant protein (Position: M1001-K1127).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747301
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human Jurkat whole cell, rat brain tissue, mouse brain tissue. IHC: human lung adenocarcinoma tissue, human ovarian serous adenocarcinoma tissue. ICC/IF: A431 cell. Flow: U87 cell, A431 cell.
Target Information
TIF1 gamma is a member of the Transcriptional Intermediary Factor 1 family, which serve as coactivators and corepressors. Its structure is similar to TIF1 alpha and TIF1 beta, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain). TIF1 gamma functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; RET-fused gene 7 protein; RING-type E3 ubiquitin transferase TRIM33; TIF 1-g; TIF1-gamma; Transcription intermediary factor 1-gamma; transcriptional intermediary factor 1 gamma; tripartite motif protein 33; tripartite motif-containing 33; Tripartite motif-containing protein 33
Gene Aliases: 8030451N04Rik; AI413936; ECTO; KIAA1113; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA; TRIM33
UniProt ID: (Human) Q9UPN9, (Mouse) Q99PP7
Entrez Gene ID: (Human) 51592, (Rat) 365894, (Mouse) 94093
DNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
co-SMAD binding
R-SMAD binding
molecular_function
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
protein ubiquitination
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
negative regulation of transcription, DNA-templated
biological_process
regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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