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Invitrogen
TIGIT Monoclonal Antibody (MBSA43), NovaFluor™ Red 700, eBioscience™
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FIGURE: 1 / 3
TIGIT Antibody (H011T03R03-A) in Flow
Normal human peripheral blood cells were unstained (left) or stained with TIGIT Monoclonal Antibody, NovaFluor Red 700 (right). All cells were co-stained with CD56 Monoclonal Antibody, eFluor 450 (Product # 48-0566-42). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora.
Product Details
H011T03R03-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
MBSA43
Conjugate
Excitation/Emission Max
637/701 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
RRID
AB_3098141 (AB_2896648)
Product Specific Information
Description
The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. MBSA43 is reported to crossreact with Rhesus macaque.
This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.
Applications Tested
This MBSA43 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.8 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Master mixes
• Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.
• We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.
Whole Blood compatibility
• When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
• See the Bulk Lysis of Human Whole Blood protocol here.
• Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.
Viability dye compatibility
• NovaFluor dyes are not compatible with DNA intercalating viability dyes.
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
CellBlox Plus Blocking Buffer
• This NovaFluor conjugate comes with CellBlox Plus Blocking Buffer (Cat. No. C001T03F01), essential for optimal staining.
• Use CellBlox Plus Blocking Buffer in all experiments with NovaFluor conjugates.
• Add 5 μL per sample to antibody cocktails/master mixes (regardless of how many Novafluor-conjugated antibodies are present) before combining with cells.
• CellBlox Plus Blocking Buffer is compatible with either Super Bright Complete Blocking Buffer or Brilliant Stain Buffer and can be used in antibody cocktails/master mixes with those reagents.
• For single-color controls, use 5 μL of CellBlox Plus Blocking Buffer per 100 μL of cell sample (10^3 to 10^8 cells).
NovaFluor conjugates are based on Phiton technology utilizing novel fluorophore-containing nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 637 nm; Emission: 701 nm; Laser: 633-640 nm (Red) Laser
Target Information
TIGIT is a 26 kDa protein that belongs to the poliovirus receptor (PVR) family. It is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but not on B cells or naive CD4+ T cells. However, TIGIT can be upregulated on naive CD4+ T cells upon activation. TIGIT interacts with members of the PVR and PVR-like families, including CD155. This interaction mediates the engagement of NK and T cells with antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR and PVR-like proteins. Binding of TIGIT to PVR leads to increased secretion of IL-10 and decreased secretion of IL-12B, promoting the generation of mature immunoregulatory dendritic cells and suppressing T cell activation. Through these interactions, TIGIT plays a significant role in modulating immune responses and maintaining immune homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule
Gene Aliases: TIGIT; VSIG9; VSTM3; WUCAM
UniProt ID: (Human) Q495A1
Entrez Gene ID: (Human) 201633
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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