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Invitrogen
TIGIT Chimeric Recombinant Rabbit Monoclonal Antibody (1B4)
FIGURE: 1 / 2
TIGIT Chimeric Antibody (MA5-48199) in WB
Western Blot analysis of TIGIT was performed using TIGIT Chimeric Monoclonal Antibody (Product # MA5-48199). RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with TIGIT Chimeric Monoclonal Antibody at 0.3 µg/mL before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Product Details
MA5-48199
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, lambda
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1B4
Immunogen
This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074642
Product Specific Information
Specificity: This antibody is specific for murine TIGIT.
Target Information
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: T-cell immunoreceptor with Ig and ITIM domains; tigit; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3
Gene Aliases: ENSMUSG00000071552; Vstm3
Entrez Gene ID: (Mouse) 100043314
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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