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Invitrogen
TIGIT Chimeric Recombinant Rabbit Monoclonal Antibody (4D4)
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FIGURE: 1 / 1
TIGIT Chimeric Antibody (MA5-48122) in ELISA
ELISA analysis of TIGIT chimeric monoclonal antibody (Product # MA5-48122) (red line) and isotype control (blue line) was performed by coating plate with TIGIT-Fc fusion protein at a concentration of 5 µg/mL. A 3-fold serial dilution from 10,000 to 0.17 ng/mL of the antibody was done. For signal detection, a 1:4,000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used. The second increase in absorbance at 1,000 ng/mL is suggestive of a possible secondary binding epitope.
Product Details
MA5-48122
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
4D4
Immunogen
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074565
Product Specific Information
Specificity: 4D4 specifically recognises TIGIT.
Preparation: Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
Target Information
TIGIT is a 26 kDa protein that belongs to the poliovirus receptor (PVR) family. It is expressed on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but not on B cells or naive CD4+ T cells. However, TIGIT can be upregulated on naive CD4+ T cells upon activation. TIGIT interacts with members of the PVR and PVR-like families, including CD155. This interaction mediates the engagement of NK and T cells with antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR and PVR-like proteins. Binding of TIGIT to PVR leads to increased secretion of IL-10 and decreased secretion of IL-12B, promoting the generation of mature immunoregulatory dendritic cells and suppressing T cell activation. Through these interactions, TIGIT plays a significant role in modulating immune responses and maintaining immune homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: T-cell immunoreceptor with Ig and ITIM domains; tigit; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3
Gene Aliases: ENSMUSG00000071552; Vstm3
Entrez Gene ID: (Mouse) 100043314
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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