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Invitrogen
TIM17 Polyclonal Antibody
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FIGURE: 1 / 12
TIM17 Antibody (PA5-95635) in ICC/IF
Immunofluorescence analysis of TIM17 in A549 cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with TIM17 polyclonal antibody (Product # PA5-95635) at a 2 µg/mL dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution and DAPI.
Product Details
PA5-95635
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human TIMM17A (LFSMIDCSMVQVRGKED).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807437
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, rat lung tissue, rat liver tissue, rat brain tissue, mouse heart tissue, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse Neuro-2a whole cell, human placenta tissue, human K562 whole cell, human T-47D whole cell, human Caco-2 whole cell, human Hela whole cell, human THP-1 whole cell, human A431 whole cell, human A549 whole cell. IHC: human liver cancer tissue, human lung cancer tissue, human mammary cancer tissue, rat lung tissue, mouse lung tissue. ICC/IF: HELA cell, A549 cell, A549 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Translocation of nuclear encoded preproteins into the mitochondrial matrix requires the coordinated action of the translocases Tom and Tim, which are located in the outer mitochondrial membrane and the inner membrane, respectively. The mitochondrial preprotein translocases of the outer membrane (Tom) is a multi-subunit protein that contains at least eight proteins: four import receptor subunits (Tom70, Tom37, Tom22, and Tom20), three small proteins (Tom7, Tom6, and Tom5), and a structural component of the outer membrane channel (Tom40). The Tom machinery involves the import receptors, which initiate the binding of cytosolically synthesized preproteins to the outer membrane, and a general import pore (GIP), which promotes the translocation of various pre-proteins into the mitochondria. The TIM channel imports nuclear-encoded mitochondrial preproteins, and it involves three proteins, Tim17, Tim23 and Tim44, which are represented at equimolar ratios. Tim17 is expressed as two isoforms Tim17a and Tim17b, which differ only in their C termini sequences, and like Tim23, these proteins are ubiquitously expressed in fetal and adult tissues. Tim17 and Tim23 are integral membrane proteins that comprise the structural elements of the inner membrane channel by which the preproteins are transferred. The Tim44, on the other hand, is a largely hydrophilic protein that recruits the matrix located Hsp70 to the site where the preprotein emerges from the Tim channel.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Inner membrane preprotein translocase Tim17a; Mitochondrial import inner membrane translocase subunit Tim17-A; mitochondrial inner membrane translocase; preprotein translocase; translocase of inner mitochondrial membrane 17a; translocator of inner mitochondrial membrane 17 kDa, a; translocator of inner mitochondrial membrane 17a; translocator of inner mitochondrial membrane a
Gene Aliases: 17kDa; MIMT17; mTim17a; TIM17; TIM17A; TIMM17; TIMM17A
UniProt ID: (Human) Q99595, (Mouse) Q9Z0V8, (Rat) O35092
Entrez Gene ID: (Human) 10440, (Mouse) 21854, (Rat) 54311
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