Proteintech

TIMM50 Polyclonal Antibody

TIMM50 Antibody in Immunocytochemistry (ICC/IF)

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TIMM50 Antibody (22229-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 22229-1-AP (TIMM50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TIMM50 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TIMM50 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22229-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TIMM50 Fusion Protein Ag17574 (190-456 aa encoded by BC121147)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GNNPVDENG AKIPDEFDND PILVQQLRRT YKYFKDYRQM IIEPTSPCLL PDPLQEPYYQ PPYTLVLELT GVLLHPEWSL ATGWRFKKRP GIETLFQQLA PLYEIVIFTS ETGMTAFPLI DSVDPHGFIS YRLFRDATRY MDGHHVKDIS CLNRDPARVV VVDCKKEAFR LQPYNGVALR PWDGNSDDRV LLDLSAFLKT IALNGVEDVR TVLEHYALED DPLAAFKQRQ SRLEQEEQQR LAELSKSNKQ NLFLGSLTSR LWPRSKQP (190-456 aa encoded by BC121147)

Target Information

TIMM50 (Mitochondrial import inner membrane translocase subunit TIM50) is an enzyme that in humans is encoded by the TIMM50 gene. Tim50 is a subunit of the Tim23 translocase complex in the inner mitochondrial membrane. Mutations in TIMM50 can lead to epilepsy, severe intellectual disability, and 3-methylglutaconic aciduria. TIMM50 expression is increased in breast cancer cells and decreased in hypertrophic hearts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

REC drives recombination to repair double-strand breaks in animal mtDNA.

The Journal of cell biology

Klucnika A,Mu P,Jezek J,McCormack M,Di Y,Bradshaw CR,Ma H

Mon Jan 02 00:00:00 UTC 2023

TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria.

Cell reports

Akabane S,Watanabe K,Kosako H,Yamashita SI,Nishino K,Kato M,Sekine S,Kanki T,Matsuda N,Endo T,Oka T

Tue May 30 00:00:00 UTC 2023

Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain.

Frontiers in cell and developmental biology

Robinson DRL,Hock DH,Muellner-Wong L,Kugapreethan R,Reljic B,Surgenor EE,Rodrigues CHM,Caruana NJ,Stroud DA

Sat Mar 19 00:00:00 UTC 2022

The Novel Non-coding Transcriptional Regulator Gm18840 Drives Cardiomyocyte Apoptosis in Myocardial Infarction Post Ischemia/Reperfusion.

Frontiers in cell and developmental biology

Luo C,Xiong S,Huang Y,Deng M,Zhang J,Chen J,Yang R,Ke X

Wed Sep 20 00:00:00 UTC 2023

Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.

Molecular & cellular proteomics : MCP

Fielden LF,Scott NE,Palmer CS,Khoo CA,Newton HJ,Stojanovski D

Mon Mar 14 00:00:00 UTC 2022

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

eLife

Le Vasseur M,Friedman J,Jost M,Xu J,Yamada J,Kampmann M,Horlbeck MA,Salemi MR,Phinney BS,Weissman JS,Nunnari J

Wed May 26 00:00:00 UTC 2021

Long non-coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma.

Cell proliferation

Guan Z,Wang Y,Wang Y,Liu X,Wang Y,Zhang W,Chi X,Dong Y,Liu X,Shao S,Zhan Q

Wed Apr 01 00:00:00 UTC 2020

Long non-coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma.

Cell proliferation

Guan Z,Wang Y,Wang Y,Liu X,Wang Y,Zhang W,Chi X,Dong Y,Liu X,Shao S,Zhan Q

Wed Apr 01 00:00:00 UTC 2020

MiR-7 mediates mitochondrial impairment to trigger apoptosis and necroptosis in Rhabdomyosarcoma.

Biochimica et biophysica acta. Molecular cell research

Yang L,Kong D,He M,Gong J,Nie Y,Tai S,Teng CB

Tue Dec 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: homolog of yeast Tim50; MGC102733; Mitochondrial import inner membrane translocase subunit TIM50; similar to translocase of inner mitochondrial membrane 50 homolog; Tim50-like protein; translocase of inner mitochondrial membrane 50 homolog

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Gene Aliases: 2810403L02Rik; AU015082; PRO1512; TIM50; TIM50L; TIMM50

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UniProt ID: (Human) Q3ZCQ8, (Mouse) Q9D880

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Entrez Gene ID: (Rat) 687295, (Human) 92609, (Mouse) 66525

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Function(s)

phosphoprotein phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity interleukin-2 receptor binding ribonucleoprotein complex binding RNA binding protein binding

Process(es)

release of cytochrome c from mitochondria protein dephosphorylation mitochondrial membrane organization protein transport peptidyl-tyrosine dephosphorylation protein import into mitochondrial matrix transport dephosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIMM50 Polyclonal Antibody

TIMM50 Antibody (22229-1-AP) in ICC/IF

Product Details

22229-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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