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Invitrogen
TIMM8A Polyclonal Antibody
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FIGURE: 1 / 12
TIMM8A Antibody (PA5-143973) in ICC/IF
Immunocytochemistry analysis of TIMM8A in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TIMM8A Polyclonal antibody (Product # PA5-143973) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate... View More
Product Details
PA5-143973
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human TIMM8A/DDP recombinant protein (Position: A14-D97).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075187
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HELA whole cell, human MCF-7 whole cell, human PC-3 whole cell, human K562 whole cell, human HEPG2 whole cell, monkey COS-7 whole cell, monkey kidney tissue, rat brain tissue, rat liver tissue, mouse liver tissue. IHC: human melanoma tissue, human testicular cancer tissue, human lung cancer tissue, human tonsil cancer tissue, human bladder cancer tissue, human gastric cancer tissue, human ovarian cancer tissue, rat brain tissue. ICC/IF: A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins. Probably necessary for normal neurologic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deafness dystonia protein 1; Deafness dystonia protein 1 homolog; deafness/dystonia peptide; Mitochondrial import inner membrane translocase subunit Tim8 A; translocase of inner mitochondrial membrane 8 homolog a; translocase of inner mitochondrial membrane 8 homolog a1; X-linked deafness dystonia protein
Gene Aliases: DDP; DDP1; DFN1; DXHXS1274E; Fci-12; MTS; TIM8; TIM8A; TIMM8A; Timm8a1
UniProt ID: (Human) O60220, (Mouse) Q9WVA2, (Rat) Q9WVA1
Entrez Gene ID: (Human) 1678, (Mouse) 30058, (Rat) 84383
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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