Invitrogen

TIMP1 Monoclonal Antibody (F31 P2 A5)

TIMP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

TIMP1 Antibody (MA1-773) in ICC/IF

Immunofluorescence analysis of TIMP1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP1 (F31 P2 A5) Mouse Monoclonal Antibody (Product # MA1-773) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

TIMP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TIMP1 Antibody — Fig. 5 KDM3A activates the transcription of Timp1 in cardiomyocytes. Cardiomyocytes (CM) and cardiac fibroblasts (cFb) were isolated from WT and Kdm3a-Tg mouse hearts at week 6 post-Sham and TAC surgery. a - e Transcripts of fetal genes and fibrosis-related ECM genes were measured by qRT-PCR, normalized against internal Gapdh, and expressed relative to WT Sham CM. n = 3 +- SEM , * , # p < 0.05 by student t test. *, WT TAC vs WT Sham. # , Tg-TAC vs WT-TAC. f Cardiomyocytes from Kdm3a -Tg mouse hearts at week 6 post-Sham and TAC surgery were isolated and used for ChIP assay with antibodies against KDM3A (left panel) or H3K9me2 (right panel). The relative occupancies of KDM3A and H3K9me2 at Timp1 promoter were normalized against Input and expressed relative to Sham control. n = 3 +- SEM , *p < 0.05 ( t test). g Timp1-Luciferase reporter was transfected in combination with plasmids as indicate into 293 T cells. Cells were harvested 48 h after transfection. Luciferase activities were normalized against co-transfected beta-galactosidase, expressed relative to vector-transfected cells. n = 3 +- SEM. (h-k) Kdm3a- Tg mice were transduced with adenovirus AAV9 expressing control or Timp1 shRNA and subjected to Sham or TAC surgery. Hearts were echoed and harvested 5 weeks post-surgery. h WB of TIMP1 and GAPDH, i HW/BW, j %FS, and k percent of fibrotic area of Sham (S), TAC (T) Kdm3a-Tg mice transduced with control or Timp1 shRNA. n = 7 +- SEM, * p < 0.05 (ANOVA) {KD}

Product Details

MA1-773

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250-1:1,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

F31 P2 A5

Immunogen

Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_889482

Product Specific Information

MA1-773 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-773 detects TIMP-1 from human samples.

MA1-773 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures. By Western blot, MA1-773 detects a ~30 kDa band representing TIMP-1 from K562 cell lysate. It is recommended to block with 3% BSA.

The MA1-773 immunogen is a synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.This sequence is 100% conserved in some non-human primate TIMP-1.

Target Information

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Remifentanil modulates the TLR4‑mediated MMP‑9/TIMP1 balance and NF‑κB/STAT3 signaling in LPS‑induced A549 cells.

Experimental and therapeutic medicine

Chang J,Zhang W

MA1-773 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that remifentanil downregulated TLR4 to reduce MMP-9/TIMP1 imbalance to inhibit inflammatory dysfunction in LPS-treated A549 cells, by regulating NF-?B/STAT3 signaling.

Wed Feb 01 00:00:00 UTC 2023

Remifentanil modulates the TLR4‑mediated MMP‑9/TIMP1 balance and NF‑κB/STAT3 signaling in LPS‑induced A549 cells.

Experimental and therapeutic medicine

Chang J,Zhang W

Wed Feb 01 00:00:00 UTC 2023

Surface-bound matrix metalloproteinase-8 on macrophages: Contributions to macrophage pericellular proteolysis and migration through tissue barriers.

Physiological reports

Wang X,Zhang D,Fucci QA,Dollery CM,Owen CA

Published figure using TIMP1 monoclonal antibody (Product # MA1-773) in Immunocytochemistry

Mon Mar 01 00:00:00 UTC 2021

A combination of pirfenidone and TGF-β inhibition mitigates cystic echinococcosis-associated hepatic injury.

Parasitology

Wang E,Liao Z,Wang L,Liao Y,Xu X,Liu P,Wang X,Hou J,Jiang H,Wu X,Chen X

MA1-773 was used in Western Blotting to reveal that the inhibitory receptors on the surface of liver NK cells were up-regulated, whereas the activating receptors were down-regulated over time.

Tue Jun 01 00:00:00 UTC 2021

Protective Effects of Kuding Tea (Ilex kudingcha C. J. Tseng) Polyphenols on UVB-Induced Skin Aging in SKH1 Hairless Mice.

Molecules (Basel, Switzerland)

Yi R,Zhang J,Sun P,Qian Y,Zhao X

MA1-773 was used in Western Blotting to study the protective effects of Kuding tea polyphenols on ultraviolet B-induced skin injury of SKH1 hairless mice.

Wed Mar 13 00:00:00 UTC 2019

Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells.

Molecules (Basel, Switzerland)

Qian Y,Song JL,Sun P,Yi R,Liu H,Feng X,Park KY,Zhao X

MA1-773 was used in Western Blotting to investigate the enhanced anti-proliferative effect of Lactobacillus casei strain Shirota on geniposide actions in human oral squamous carcinoma HSC-3 cells.

Thu May 03 00:00:00 UTC 2018

Histone lysine dimethyl-demethylase KDM3A controls pathological cardiac hypertrophy and fibrosis.

Nature communications

Zhang QJ,Tran TAT,Wang M,Ranek MJ,Kokkonen-Simon KM,Gao J,Luo X,Tan W,Kyrychenko V,Liao L,Xu J,Hill JA,Olson EN,Kass DA,Martinez ED,Liu ZP

MA1-773 was used in Chromatin immunoprecipitation to investigate gain and loss of function of a H3K9me2 specific demethylase, Kdm3a, and show it promotes left ventricular hypertrophy and fibrosis in response to pressure-overload.

Fri Dec 07 00:00:00 UTC 2018

Intranasal Curcumin Inhibits Pulmonary Fibrosis by Modulating Matrix Metalloproteinase-9 (MMP-9) in Ovalbumin-Induced Chronic Asthma.

Inflammation

Chauhan PS,Dash D,Singh R

MA1773 was used in western blot to study the effect of intranasal curcumin on airway remodeling and fibrosis in murine model of chronic asthma

Wed Feb 01 00:00:00 UTC 2017

Articular cartilage degradation is prevented by tanshinone IIA through inhibiting apoptosis and the expression of inflammatory cytokines.

Molecular medicine reports

Jia PT,Zhang XL,Zuo HN,Lu X,Li L

MA1-773 was used in Western Blotting to investigate the effect of tanshinone IIA on the degradation of articular cartilage in a rat model of osteoarthritis.

Wed Nov 01 00:00:00 UTC 2017

Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B

MA1773 was used in western blot to infer a RANKL-dependent osteoclastogenesis after debridement of osteomyelitis coinciding with elevated B cells and simultaneously decreased osteogenesis

Wed Nov 01 00:00:00 UTC 2017

Inhibition of transforming growth factor beta signaling by halofuginone as a modality for pancreas fibrosis prevention.

Pancreas

Zion O,Genin O,Kawada N,Yoshizato K,Roffe S,Nagler A,Iovanna JL,Halevy O,Pines M

MA1-773 was used in immunohistochemistry to study the mechanism by which halofuginone prevents cerulein-induced pancreatic fibrosis

Fri May 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: Collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metalloproteinase-1; Tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1

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Gene Aliases: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1; TPA-S1

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UniProt ID: (Rhesus monkey) Q95KL9, (Human) P01033, (Mouse) P12032

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Entrez Gene ID: (Rhesus monkey) 574348, (Human) 7076, (Mouse) 21857

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Function(s)

protease binding cytokine activity protein binding growth factor activity metalloendopeptidase inhibitor activity metal ion binding enzyme inhibitor activity peptidase inhibitor activity

Process(es)

cell activation platelet degranulation aging positive regulation of cell proliferation response to hormone negative regulation of endopeptidase activity extracellular matrix disassembly response to cytokine wound healing negative regulation of apoptotic process negative regulation of catalytic activity response to peptide hormone negative regulation of membrane protein ectodomain proteolysis cartilage development negative regulation of trophoblast cell migration regulation of integrin-mediated signaling pathway negative regulation of peptidase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TIMP1 Monoclonal Antibody (F31 P2 A5)

TIMP1 Antibody (MA1-773) in ICC/IF

Product Details

MA1-773

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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