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Invitrogen
TLE1 Monoclonal Antibody (OTI1H2)
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FIGURE: 1 / 24
TLE1 Antibody (MA5-26424) in ICC/IF
Immunofluorescence analysis of TLE1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TLE1 Monoclonal Antibody (OTI1H2) (Product # MA5-26424) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product ... View More
Product Details
MA5-26424
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1H2
Immunogen
Human recombinant protein fragment corresponding to amino acids 180-460 of TLE1 produced in E.coli
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2725526
Target Information
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E(Sp1) homolog; Enhancer of split groucho-like protein 1; ESG1; RP11-203D19.1; TLE 1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); Transducin-like enhancer protein 1
Gene Aliases: ESG; ESG1; GRG1; TLE1
UniProt ID: (Human) Q04724
Entrez Gene ID: (Human) 7088
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
signal transduction
multicellular organism development
organ morphogenesis
positive regulation of gene expression
Wnt signaling pathway
negative regulation of Wnt signaling pathway
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of transcription, DNA-templated
beta-catenin-TCF complex assembly
negative regulation of anoikis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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