Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
TLE4 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 3
TLE4 Antibody (PA5-30192) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TLE4 was performed in DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE4 Polyclonal Antibody (Product # PA5-30192) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-30192
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.95 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547666
Product Specific Information
Recommended positive controls: NT2D1, IMR32, Jurkat, HeLa.
Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: B lymphocyte gene 1; enhancer of split groucho 4; Grg-4; Groucho-related protein 4; Protein ESP2; transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 4, E(spl) homolog; transducin-like enhancer of split 4, homolog of Drosophila E(sp1); transducin-like enhancer of split 4, homolog of Drosophila E(spl); Transducin-like enhancer protein 4
Gene Aliases: BCE-1; BCE1; E(spI); ESG; ESG4; Esp2; Grg-4; GRG4; KIAA1261; TLE4
UniProt ID: (Human) Q04727, (Rat) Q07141
Entrez Gene ID: (Human) 7091, (Rat) 25565
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
biological_process
Wnt signaling pathway
beta-catenin-TCF complex assembly
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
negative regulation of nucleic acid-templated transcription
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support