Invitrogen

TLR2 Recombinant Rabbit Monoclonal Antibody (JM22-41)

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1 Published Figure
9 References
View all (75) TLR2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-32787

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 3 publications 3 publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:100
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM22-41

Immunogen

Recombinant protein within Human TLR2 aa 21-588
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2810063

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2; Toll/interleukin-1 receptor-like protein 4

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Gene Aliases: CD282; Ly105; TIL4; TLR2

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UniProt ID: (Human) O60603, (Mouse) Q9QUN7

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Entrez Gene ID: (Human) 7097, (Rat) 310553, (Mouse) 24088

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Function(s)
lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity diacyl lipopeptide binding lipoteichoic acid binding lipopeptide binding
Process(es)
response to hypoxia microglial cell activation toll-like receptor signaling pathway response to molecule of fungal origin positive regulation of leukocyte migration cell surface pattern recognition receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway leukotriene metabolic process inflammatory response immune response I-kappaB phosphorylation negative regulation of cell proliferation response to bacterium response to toxic substance positive regulation of Wnt signaling pathway central nervous system myelin formation response to bacterial lipoprotein response to peptidoglycan response to lipopolysaccharide response to progesterone negative regulation of interleukin-12 production negative regulation of interleukin-17 production positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production response to insulin positive regulation of toll-like receptor signaling pathway toll-like receptor 2 signaling pathway positive regulation of NF-kappaB import into nucleus detection of triacyl bacterial lipopeptide detection of diacyl bacterial lipopeptide positive regulation of tumor necrosis factor biosynthetic process chloramphenicol transport negative regulation of growth of symbiont in host innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of oligodendrocyte differentiation positive regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process induction by symbiont of defense-related host nitric oxide production positive regulation of macrophage cytokine production response to fatty acid cellular response to bacterial lipopeptide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide response to molecule of bacterial origin cytokine secretion involved in immune response immune system process regulation of dendritic cell cytokine production signal transduction interleukin-10 production regulation of cytokine secretion apoptotic process positive regulation of gene expression lipopolysaccharide-mediated signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway
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