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Invitrogen
TLR2 Monoclonal Antibody (TL2.1)
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FIGURE: 1 / 7
TLR2 Antibody (MA5-16200) in Flow
Flow cytometry of TLR2 in stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green). Samples were incubated in TLR2 monoclonal antibody (Product # MA5-16200) using a dilution of 1 µg/10^6 cells. Image using the FITC form of this antibody.
Product Details
MA5-16200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rabbit,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
TL2.1
Immunogen
CHO cells transfected with human TLR2 cDNA
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2537718
Product Specific Information
Suggested positive control: antigen standard for TLR2 (transient overexpression lysate), PBMCs, Ramos.
Target Information
TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD282; TLR-2; Toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2; Toll/interleukin-1 receptor-like protein 4
Gene Aliases: CD282; Ly105; TIL4; TLR2
UniProt ID: (Human) O60603, (Mouse) Q9QUN7
Entrez Gene ID: (Dog) 448807, (Human) 7097, (Rabbit) 100009578, (Rat) 310553, (Mouse) 24088
transmembrane signaling receptor activity
lipopolysaccharide binding
lipopolysaccharide receptor activity
receptor activity
protein binding
signaling pattern recognition receptor activity
Toll-like receptor binding
triacyl lipopeptide binding
peptidoglycan binding
protein heterodimerization activity
diacyl lipopeptide binding
lipoteichoic acid binding
lipopeptide binding
response to hypoxia
microglial cell activation
toll-like receptor signaling pathway
response to molecule of fungal origin
positive regulation of leukocyte migration
cell surface pattern recognition receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
leukotriene metabolic process
inflammatory response
immune response
I-kappaB phosphorylation
negative regulation of cell proliferation
response to bacterium
response to toxic substance
positive regulation of Wnt signaling pathway
central nervous system myelin formation
response to bacterial lipoprotein
response to peptidoglycan
response to lipopolysaccharide
response to progesterone
negative regulation of interleukin-12 production
negative regulation of interleukin-17 production
positive regulation of chemokine production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
response to insulin
positive regulation of toll-like receptor signaling pathway
toll-like receptor 2 signaling pathway
positive regulation of NF-kappaB import into nucleus
detection of triacyl bacterial lipopeptide
detection of diacyl bacterial lipopeptide
positive regulation of tumor necrosis factor biosynthetic process
chloramphenicol transport
negative regulation of growth of symbiont in host
innate immune response
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
nitric oxide metabolic process
positive regulation of oligodendrocyte differentiation
positive regulation of cytokine secretion
positive regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
positive regulation of nitric-oxide synthase biosynthetic process
induction by symbiont of defense-related host nitric oxide production
positive regulation of macrophage cytokine production
response to fatty acid
cellular response to bacterial lipopeptide
cellular response to lipoteichoic acid
cellular response to peptidoglycan
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
regulation of cytokine secretion
response to molecule of bacterial origin
cytokine secretion involved in immune response
immune system process
regulation of dendritic cell cytokine production
signal transduction
interleukin-10 production
apoptotic process
positive regulation of gene expression
lipopolysaccharide-mediated signaling pathway
toll-like receptor TLR1:TLR2 signaling pathway
toll-like receptor TLR6:TLR2 signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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