Invitrogen

TLR4 Monoclonal Antibody (HTA125)

1 Published Figure

6 References

View all (66) TLR4 (CD284) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (1)

FIGURE: 1 / 3

TLR4 Antibody (MA1-80122) in Flow

Flow Cytometry analysis of TLR4 in formaldehyde-fixed THP-1 cells (green) compared to an isotype control (blue). Staining was performed using a TLR4 monoclonal antibody (Product # MA1-80122) at a dilution of 2µg x 10^6 cells., followed by secondary detection using a Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate (Product # A-21202) at a dilution of 1:200. Mouse IgG2a Isotype Control (Product # MA5-14441) was used as a negative control at the same dilution as the primary antibody. Data provided courtesy... View More

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Product Details

MA1-80122

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1:10-1:50

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 3 publications 3 publications

Functional Assay (Functional)

Assay-dependent

Product Specifications

Species Reactivity

Dog, Guinea pig, Human, Pig, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

HTA125

Immunogen

Ba/F3 cell line expressing TLR4 (CD284).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_928719

Product Specific Information

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

In vitro evidence against productive SARS-CoV-2 infection of human testicular cells: Bystander effects of infection mediate testicular injury.

PLoS pathogens

Giannakopoulos S,Strange DP,Jiyarom B,Abdelaal O,Bradshaw AW,Nerurkar VR,Ward MA,Bakse J,Yap J,Vanapruks S,Boisvert WA,Tallquist MD,Shikuma C,Sadri-Ardekani H,Clapp P,Murphy SV,Verma S

Published figure using TLR4 monoclonal antibody (Product # MA1-80122) in Neutralization

Mon May 01 00:00:00 UTC 2023

The protease inhibitor cystatin C down-regulates the release of IL-β and TNF-α in lipopolysaccharide activated monocytes.

Journal of leukocyte biology

Gren ST,Janciauskiene S,Sandeep S,Jonigk D,Kvist PH,Gerwien JG,Håkansson K,Grip O

MA180122 was used in flow cytometry to assess the ability of human cystatin C to alter pathogenic activated monocytes and modulate Crohn's disease

Sat Oct 01 00:00:00 UTC 2016

Elevated Toll-Like Receptor-Induced CXCL8 Secretion in Human Blood Basophils from Allergic Donors Is Independent of Toll-Like Receptor Expression Levels.

PloS one

Steiner M,Hawranek T,Schneider M,Ferreira F,Horejs-Hoeck J,Harrer A,Himly M

MA180122 was used in flow cytometry to assess expression levels of Toll-like receptor 1, 2, 4 and 6 on purified basophils from birch pollen-, house dust mite-, and non-allergic individuals

Tue Jul 26 00:00:00 UTC 2016

Dysfunctional B-cell activation in cirrhosis resulting from hepatitis C infection associated with disappearance of CD27-positive B-cell population.

Hepatology (Baltimore, Md.)

Doi H,Iyer TK,Carpenter E,Li H,Chang KM,Vonderheide RH,Kaplan DE

MA1-80122 was used in blocking or activating experiment to study the role of hepatitis C infection in the B-cell dysfunction in cirrhosis

Thu Mar 01 00:00:00 UTC 2012

IFN-gamma primes intact human coronary arteries and cultured coronary smooth muscle cells to double-stranded RNA- and self-RNA-induced inflammatory responses by upregulating TLR3 and melanoma differentiation-associated gene 5.

Journal of immunology (Baltimore, Md. : 1950)

Ahmad U,Ali R,Lebastchi AH,Qin L,Lo SF,Yakimov AO,Khan SF,Choy JC,Geirsson A,Pober JS,Tellides G

MA1-80122 was used in blocking or activating experiment to investigate the contribution of RNA to the inflammatory milieu of arteriosclerosis

Thu Jul 15 00:00:00 UTC 2010

Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes.

Journal of leukocyte biology

Tunheim G,Schjetne KW,Fredriksen AB,Sandlie I,Bogen B

MA1-80122 was used in flow cytometry to assess targeting CD14 using recombinant Ig-based vaccines in order to elicit human T cell responses

Tue Mar 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: CD284; homolog of Drosophila toll; hToll; Toll-like receptor 4; Toll-like receptor4 protein

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Gene Aliases: ARMD10; CD284; EGK_07381; hToll; TLR-4; TLR4; TOLL

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UniProt ID: (Human) O00206

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Entrez Gene ID: (Rhesus monkey) 574360, (Pig) 399541, (Human) 7099, (Dog) 403417

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Function(s)

lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TLR4 Monoclonal Antibody (HTA125)

TLR4 Antibody (MA1-80122) in Flow

Product Details

MA1-80122

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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