Proteintech

TMEM119 Polyclonal Antibody

TMEM119 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

TMEM119 Antibody (27585-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 27585-1-AP) TMEM119 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

Product Details

27585-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:800

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TMEM119 Fusion Protein Ag26269 (119-218 aa encoded by NM_181724)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

27585-1-AP recognizes a 45-50 kDa band likely due to glycosylation.

Immunogen sequence: MRQKQKASA YYPSSFPKKK YVDQSDRAGG PRAFSEVPDR APDSRPEEAL DSSRQLQADI LAATQNLKSP TRAALGGGDG ARMVEGRGAE EEEKGSQEGD QE (119-218 aa encoded by NM_181724)

Target Information

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway.

Neurochemical research

Wei X,Huang C,Chen K,Liu S,Wang M,Yang L,Wang Y

Fri Sep 01 00:00:00 UTC 2023

CD34⁺ cell-derived fibroblast-macrophage cross-talk drives limb ischemia recovery through the OSM-ANGPTL signaling axis.

Science advances

Song Y,Yang J,Li T,Sun X,Lin R,He Y,Sun K,Han J,Yang G,Li X,Liu B,Yang D,Dang G,Ma X,Du X,Zhang B,Hu Y,Kong W,Wang X,Zhang H,Xu Q,Feng J

Fri Apr 14 00:00:00 UTC 2023

Transmembrane protein 119 is neither a specific nor a reliable marker for microglia.

Glia

Vankriekelsvenne E,Chrzanowski U,Manzhula K,Greiner T,Wree A,Hawlitschka A,Llovera G,Zhan J,Joost S,Schmitz C,Ponsaerts P,Amor S,Nutma E,Kipp M,Kaddatz H

Wed Jun 01 00:00:00 UTC 2022

A modified high-yield method for primary culture of rat retinal microglial cells.

Experimental eye research

Han X,Liu X,Zhuang J,Liang X,Luo Q,Chen P,Wen Y,Wang X,Peng J,Yang Y,Sha X,Zhuang J

Tue Feb 01 00:00:00 UTC 2022

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Science advances

Guerrero-Juarez CF,Lee GH,Liu Y,Wang S,Karikomi M,Sha Y,Chow RY,Nguyen TTL,Iglesias VS,Aasi S,Drummond ML,Nie Q,Sarin K,Atwood SX

Fri Jun 10 00:00:00 UTC 2022

Didymin Suppresses Microglia Pyroptosis and Neuroinflammation Through the Asc/Caspase-1/GSDMD Pathway Following Experimental Intracerebral Hemorrhage.

Frontiers in immunology

Gu L,Sun M,Li R,Zhang X,Tao Y,Yuan Y,Luo X,Xie Z

Mon Mar 14 00:00:00 UTC 2022

Hyperphosphorylated tau mediates neuronal death by inducing necroptosis and inflammation in Alzheimer's disease.

Journal of neuroinflammation

Dong Y,Yu H,Li X,Bian K,Zheng Y,Dai M,Feng X,Sun Y,He Y,Yu B,Zhang H,Wu J,Yu X,Wu H,Kong W

Mon Aug 15 00:00:00 UTC 2022

Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases.

Frontiers in cellular neuroscience

Banerjee A,Lu Y,Do K,Mize T,Wu X,Chen X,Chen J

Sat May 04 00:00:00 UTC 2024

MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.

Pharmacological research

Xin D,Li T,Chu X,Ke H,Liu D,Wang Z

Mon Feb 01 00:00:00 UTC 2021

P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.

Nature communications

Yeini E,Ofek P,Pozzi S,Albeck N,Ben-Shushan D,Tiram G,Golan S,Kleiner R,Sheinin R,Israeli Dangoor S,Reich-Zeliger S,Grossman R,Ram Z,Brem H,Hyde TM,Magod P,Friedmann-Morvinski D,Madi A,Satchi-Fainaro R

Fri Mar 26 00:00:00 UTC 2021

Toll-like receptor-9 (TLR-9) deficiency alleviates optic nerve injury (ONI) by inhibiting inflammatory response in vivo and in vitro.

Experimental cell research

Zhang L,Li X

Sun Nov 01 00:00:00 UTC 2020

Toll-like receptor-9 (TLR-9) deficiency alleviates optic nerve injury (ONI) by inhibiting inflammatory response in vivo and in vitro.

Experimental cell research

Zhang L,Li X

Sun Nov 01 00:00:00 UTC 2020

Early Histone Deacetylase Inhibition Mitigates Ischemia/Reperfusion Brain Injury by Reducing Microglia Activation and Modulating Their Phenotype.

Frontiers in neurology

Li S,Lu X,Shao Q,Chen Z,Huang Q,Jiao Z,Huang X,Yue M,Peng J,Zhou X,Chao D,Zhao H,Ji J,Ji Y,Ji Q

Thu Oct 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: OBIF; Osteoblast induction factor; Transmembrane protein 119

View more View less

Gene Aliases: AW208946; BC025600; OBIF; PSEC0199; TMEM119; UNQ731/PRO1415

View more View less

UniProt ID: (Human) Q4V9L6, (Mouse) Q8R138

View more View less

Entrez Gene ID: (Human) 338773, (Mouse) 231633

View more View less

Function(s)

molecular_function protein binding

Process(es)

osteoblast differentiation spermatogenesis biological_process positive regulation of bone mineralization biomineral tissue development positive regulation of osteoblast proliferation positive regulation of osteoblast differentiation spermatid differentiation positive regulation of bone development ossification cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

TMEM119 Polyclonal Antibody

TMEM119 Antibody (27585-1-AP) in IHC (P)

Product Details

27585-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!