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Invitrogen
TMEM27 Polyclonal Antibody
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TMEM27 Antibody (PA5-88721) in WB
Western blot analysis of TMEM27 in extracts of various cell lines. Samples were incubated with TMEM27 Polyclonal antibody (Product # PA5-88721) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88721
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human TMEM27 (NP_0657161)
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805089
Product Specific Information
Immunogen sequence: MLWLLFFLVT AIHAELCQPG AENAFKVRLS IRTALGDKAY AWDTNEEYLF KAMVAFSMRK VPNREATEIS HVLLCNVTQR VSFWFVVTDP SKNHTLPAVE VQSAIRMNKN RINNAFFLND QTLEFLKIPS TLAPPMDPSV PIWIIIFGVI FCIIIVAIAL LILSGIWQRR RKNKEPSEVD DAEDKCENMI TIENGIPSDP LDMKGGHIND AFMTEDERLT PL; Positive Samples: Mouse Kidney, Mouse Pancreas, Rat Kidney; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
TMEM27 (Transmembrane protein 27) is also named as collectrin and Belongs to the TMEM27 family. It is a stimulator of beta cell replication and a membrane protein cleaved and shed by pancreatic beta cells that has been proposed as a beta cell mass biomarker. Western blot analysis of mouse collecting duct cell lines detected Tmem27 at an apparent molecular mass of 32 kDa. TMEM27 is an N-glycosylated protein and forms dimers, with MW of 64-75 kDa.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collectrin; kidney-specific membrane protein; Transmembrane protein 27
Gene Aliases: 0610008J07Rik; Cltrn; collectrin; NX-17; NX17; Tmem27
UniProt ID: (Rat) Q9ESG3, (Mouse) Q9ESG4
Entrez Gene ID: (Rat) 57395, (Mouse) 57394
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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