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TMEM37 Antibody (PA5-116053) in ICC/IF
Immunocytochemistry analysis of TMEM37 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116053) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TMEM37(Accession Q8WXS4), corresponding to amino acid residues M1-S42.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900687
Product Specific Information
Antibody detects endogenous levels of total TMEM37.
Target Information
Thought to stabilize the calcium channel in an inactivated (closed) state. Modulates calcium current when coexpressed with CACNA1G.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neuronal voltage-gated calcium channel gamma-like subunit; protein distantly related to to the gamma subunit family; TMEM37; tmem37 {ECO:0000250|UniProtKB:Q9JJV3}; Transmembrane protein 37; voltage-dependent calcium channel gamma subunit-like protein; Voltage-dependent calcium channel gamma-like subunit
Gene Aliases: AI173373; AW540203; Cacng5; PR; PR1; TMEM37
UniProt ID: (Human) Q8WXS4, (Rat) Q8VHW1, (Mouse) Q9JJV3
Entrez Gene ID: (Human) 140738, (Rat) 245953, (Mouse) 170706
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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