Invitrogen

TMEM66 Polyclonal Antibody

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TMEM66 Antibody (PA5-24237) in WB

Western blot analysis of TMEM66 in mouse cerebellum tissue lysates. Samples were incubated with TMEM66 polyclonal antibody (Product # PA5-24237). Lysates: 35 µg/lane. TMEM66 protein (arrow).

TMEM66 Antibody in Flow Cytometry (Flow)

TMEM66 Antibody in Immunocytochemistry, Proximity Ligation Assay (PLA) (ICC/IF, PLA)

TMEM66 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

TMEM66 Antibody (PA5-24237) in — Figure 3 Dynamic interaction of SARAF with Orai1 and STIM1. MEG-01 cells were suspended in HBS containing 300 muM CaCl 2 and were left untreated or stimulated with TG (1 muM) for 30 or 120 s. The cells were then fixed with 4% ice-cold paraformaldehyde and blocked using blocking solution from the Duolink In Situ Red kit (r) from Sigma. Samples were incubated with primary antibodies: rabbit anti-SARAF and mouse anti-STIM1 or mouse anti-Orai1 (SAB4200273, Sigma) antibodies. A Duolink assay was subsequently performed according to the manufacturer's instructions as described in Material and Methods. After performing the PLA procedure, images were taken with an Eclipse TE300 fluorescence microscope. Red spots represent individual dimers. The positive control is shown in the lower right corner. The bars indicate 10 mum. The bar graphs indicate association between SARAF and STIM1 or Orai1 expressed as the mean +- S.E.M. of 100-200 cells. The quantification of PLA signals was performed using ImageJ software. ***p < 0.001.

Product Details

PA5-24237

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1:10-1:50

Immunoprecipitation (IP)

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 33-62 amino acids from the N-terminal region of human TMEM66

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2541737

Target Information

Saraf encodes a protein that is a negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)-dependent SOCE activity: possibly act by facilitating the de-oligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARAF and Orai1 Contribute to Endothelial Cell Activation and Angiogenesis.

Frontiers in cell and developmental biology

Galeano-Otero I,Del Toro R,Khatib AM,Rosado JA,Ordóñez-Fernández A,Smani T

PA5-24237 was used in Immunohistochemistry to show that SOCE inhibition with GSK-7975A blocks aorta sprouting, as well as human umbilical vein endothelial cell (HUVEC) tube formation and migration.

Fri May 07 00:00:00 UTC 2021

SARAF and Orai1 Contribute to Endothelial Cell Activation and Angiogenesis.

Frontiers in cell and developmental biology

Galeano-Otero I,Del Toro R,Khatib AM,Rosado JA,Ordóñez-Fernández A,Smani T

PA5-24237 was used in Immunohistochemistry to show that SOCE inhibition with GSK-7975A blocks aorta sprouting, as well as human umbilical vein endothelial cell (HUVEC) tube formation and migration.

Fri May 07 00:00:00 UTC 2021

SARAF and Orai1 Contribute to Endothelial Cell Activation and Angiogenesis.

Frontiers in cell and developmental biology

Galeano-Otero I,Del Toro R,Khatib AM,Rosado JA,Ordóñez-Fernández A,Smani T

PA5-24237 was used in Immunohistochemistry to show that SOCE inhibition with GSK-7975A blocks aorta sprouting, as well as human umbilical vein endothelial cell (HUVEC) tube formation and migration.

Fri May 07 00:00:00 UTC 2021

SARAF and EFHB Modulate Store-Operated Ca²⁺ Entry and Are Required for Cell Proliferation, Migration and Viability in Breast Cancer Cells.

Cancers

Jardin I,Nieto-Felipe J,Alvarado S,Diez-Bello R,Lopez JJ,Salido GM,Smani T,Rosado JA

Published figure using TMEM66 polyclonal antibody (Product # PA5-24237) in Western Blot

Thu Aug 19 00:00:00 UTC 2021

Saraf-dependent activation of mTORC1 regulates cardiac growth.

Journal of molecular and cellular cardiology

Sanlialp A,Schumacher D,Kiper L,Varma E,Riechert E,Ho TC,Hofmann C,Kmietczyk V,Zimmermann F,Dlugosz S,Wirth A,Gorska AA,Burghaus J,Camacho Londoño JE,Katus HA,Doroudgar S,Freichel M,Völkers M

PA5-24237 was used in Western Blotting to determine the role of the store-operated calcium entry-associated regulatory factor (Saraf), encoded by the Tmem66 gene, on cardiac growth control in vitro and in vivo.

Wed Apr 01 00:00:00 UTC 2020

STIM1 phosphorylation at Y³¹⁶ modulates its interaction with SARAF and the activation of SOCE and I_CRAC.

Journal of cell science

Lopez E,Frischauf I,Jardin I,Derler I,Muik M,Cantonero C,Salido GM,Smani T,Rosado JA,Redondo PC

PA5-24237 was used in Western Blot to provide evidence supporting the idea that phosphorylation of stromal interaction molecule 1 at Y316 plays a relevant functional role in the activation and modulation of store-operated Ca2+ entry.

Wed May 15 00:00:00 UTC 2019

Role of STIM1 in the surface expression of SARAF.

Channels (Austin, Tex.)

Albarran L,Regodón S,Salido GM,Lopez JJ,Rosado JA

PA524237 was used in western blot to elucidate how SARAF influences the location of STIM1

Mon Jan 02 00:00:00 UTC 2017

Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry.

Scientific reports

Albarran L,Lopez JJ,Amor NB,Martin-Cano FE,Berna-Erro A,Smani T,Salido GM,Rosado JA

Published figure using TMEM66 polyclonal antibody (Product # PA5-24237) in Immunoprecipitation

Tue Apr 12 00:00:00 UTC 2016

Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry.

Scientific reports

Albarran L,Lopez JJ,Amor NB,Martin-Cano FE,Berna-Erro A,Smani T,Salido GM,Rosado JA

Published figure using TMEM66 polyclonal antibody (Product # PA5-24237) in Immunoprecipitation

Tue Apr 12 00:00:00 UTC 2016

Dynamic interaction of SARAF with STIM1 and Orai1 to modulate store-operated calcium entry.

Scientific reports

Albarran L,Lopez JJ,Amor NB,Martin-Cano FE,Berna-Erro A,Smani T,Salido GM,Rosado JA

Published figure using TMEM66 polyclonal antibody (Product # PA5-24237) in Immunoprecipitation

Tue Apr 12 00:00:00 UTC 2016

Translocation between PI(4,5)P2-poor and PI(4,5)P2-rich microdomains during store depletion determines STIM1 conformation and Orai1 gating.

Nature communications

Maléth J,Choi S,Muallem S,Ahuja M

PA5-24237 was used in western blot to determine STIM1 conformation and Orai1 gating by translocation between PI(4,5)P2-poor and PI(4,5)P2-rich microdomains during store depletion

Wed Dec 17 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: HBV X-transactivated gene 3 protein; HBV XAg-transactivated protein 3; Protein FOAP-7; SARAF; SARAF long isoform; SARAF short isoform; SOCE-associated regulatory factor; Store-operated calcium entry-associated regulatory factor; testicular secretory protein Li 59; Transmembrane protein 66

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Gene Aliases: 1810045K07Rik; FOAP-7; HSPC035; NPD003; PSEC0019; SARAF; TMEM66; UNQ1967/PRO4499; XTP3

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UniProt ID: (Human) Q96BY9, (Mouse) Q8R3Q0

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Entrez Gene ID: (Human) 51669, (Mouse) 67887

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Function(s)

protein binding

Process(es)

calcium ion transport regulation of store-operated calcium entry transport ion transport

Performance Guarantee

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TMEM66 Polyclonal Antibody

TMEM66 Antibody (PA5-24237) in WB

Product Details

PA5-24237

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

in situ PLA (PLA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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