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TMP21 Antibody (BS-11262R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with TMP21 Polyclonal Antibody, Unconjugated (bs-11262R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product Details
BS-11262R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human TMP21, amino acids 101-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the -gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the -gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer's disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased -gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate -gamma-secretase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 21 kDa transmembrane trafficking protein; 21 kDa transmembrane-trafficking protein; integral membrane protein Tmp21-I (p23); p23; p24 family protein delta-1; p24delta; p24delta1; Protein TMED10; S31I125; S31III125; testicular tissue protein Li 206; Tmp-21-I; Transmembrane emp24 domain-containing protein 10; transmembrane emp24-like trafficking protein 10; Transmembrane protein Tmp21; transmembrane trafficking protein; transmembrane trafficking protein 21
Gene Aliases: 1110014C03Rik; p23; P24(DELTA); p24d1; p24delta1; S31I125; S31III125; TMED10; Tmp-21-I; TMP21
UniProt ID: (Human) P49755, (Rat) Q63584, (Mouse) Q9D1D4
Entrez Gene ID: (Human) 10972, (Rat) 84599, (Mouse) 68581
kidney development
intracellular protein transport
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
Golgi organization
cargo loading into vesicle
COPI-coated vesicle budding
response to alkaloid
regulated exocytosis
vesicle targeting, to, from or within Golgi
COPI coating of Golgi vesicle
COPII vesicle coating
protein oligomerization
regulation of beta-amyloid formation
vesicle targeting
transport
protein transport
vesicle-mediated transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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