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TMSB4X Polyclonal Antibody

TMSB4X Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TMSB4X Antibody (19850-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (TMSB4X antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

TMSB4X Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

TMSB4X Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

19850-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:800

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TMSB4X Fusion Protein Ag13914 (1-63 aa encoded by BC001631)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: AQTRLRSYS CASLRFSSAT MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES (1-63 aa encoded by BC001631)

Target Information

Thymosin-beta4 is a small, actin-sequestering protein belonging to the thymosin-beta family that is found at high concentrations within the spleen, thymus, and peritoneal macrophages, where it is most notably responsible for the organization of cytoskeletal structure. In mammalian tissues, this protein acts as a modulator for the polymerization/depolymerization of actin through the formation of a 1:1 complex with the monomer G (globular)-actin, and inhibits actin's polymerization to form F (filamentous) actin, which together with other proteins binds microfilaments to construct the cytoskeleton. Commonly found at significant quantities within the brain, lungs, liver, kidneys, testes, and heart, thymosin-beta4 has also been shown to be synthesized by cells unrelated to the reticuloendothelial system, such as myoblasts and fibroblasts, and expressed at irregular levels by several hemopoietic cell lines, malignant lymphoid cells and myeloma cells. In addition to regulating actin polymerization, research has also found thymosin-beta4 to stimulate the secretion of hypothalamic luteinizing hormone-releasing hormone and luteinizing hormone, inhibit the migration of peritoneal macrophages, induce phenotypic changes in T cell lines during early host defense mechanisms, and inhibit the progression of hematopoietic pluripotent stem cells into the S-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lysine-40 succinylation of TAGLN2 induces glioma angiogenesis and tumor growth through regulating TMSB4X.

Cancer gene therapy

Zhang X,Han J,Fan D,Wang J,Lin X,Zhang H,Zhang C,Bai J,Huang H,Gu Y

Sun Jan 01 00:00:00 UTC 2023

Lysine-40 succinylation of TAGLN2 induces glioma angiogenesis and tumor growth through regulating TMSB4X.

Cancer gene therapy

Zhang X,Han J,Fan D,Wang J,Lin X,Zhang H,Zhang C,Bai J,Huang H,Gu Y

Sun Jan 01 00:00:00 UTC 2023

Plasma proteome profiling using tandem mass tag labeling technology reveals potential biomarkers for Parkinson's disease: a preliminary study.

Proteomics. Clinical applications

Zhao Y,Zhang Y,Zhang J,Yang G

Tue Mar 01 00:00:00 UTC 2022

Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.

Haematologica

Scheller I,Beck S,Göb V,Gross C,Neagoe RAI,Aurbach K,Bender M,Stegner D,Nagy Z,Nieswandt B

Thu Dec 01 00:00:00 UTC 2022

Tβ4 ameliorates oxidative damage and apoptosis through ERK/MAPK and 5-HT1A signaling pathway in Aβ insulted SH-SY5Y cells.

Life sciences

Zhang GH,Pare RB,Chin KL,Qian YH

Thu Nov 25 00:00:00 UTC 2021

Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma.

Nature communications

Pu W,Shi X,Yu P,Zhang M,Liu Z,Tan L,Han P,Wang Y,Ji D,Gan H,Wei W,Lu Z,Qu N,Hu J,Hu X,Luo Z,Li H,Ji Q,Wang J,Zhang X,Wang YL

Mon Oct 18 00:00:00 UTC 2021

Single-cell proteomics reveals changes in expression during hair-cell development.

eLife

Zhu Y,Scheibinger M,Ellwanger DC,Krey JF,Choi D,Kelly RT,Heller S,Barr-Gillespie PG

Mon Nov 04 00:00:00 UTC 2019

Thymosin β4 suppresses CCl₄ -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.

The journal of gene medicine

Li H,Li Q,Zhang X,Zheng X,Zhang Q,Hao Z

Sat Sep 01 00:00:00 UTC 2018

Thymosin β4 suppresses CCl₄ -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.

The journal of gene medicine

Li H,Li Q,Zhang X,Zheng X,Zhang Q,Hao Z

Sat Sep 01 00:00:00 UTC 2018

Thymosin β4 suppresses CCl₄ -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.

The journal of gene medicine

Li H,Li Q,Zhang X,Zheng X,Zhang Q,Hao Z

Sat Sep 01 00:00:00 UTC 2018

Thymosin β4 suppresses CCl₄ -induced murine hepatic fibrosis by down-regulating transforming growth factor β receptor-II.

The journal of gene medicine

Li H,Li Q,Zhang X,Zheng X,Zhang Q,Hao Z

Sat Sep 01 00:00:00 UTC 2018

Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.

Scientific reports

Chi LH,Chang WM,Chang YC,Chan YC,Tai CC,Leung KW,Chen CL,Wu AT,Lai TC,Li YJ,Hsiao M

Tue Aug 22 00:00:00 UTC 2017

Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.

Scientific reports

Chi LH,Chang WM,Chang YC,Chan YC,Tai CC,Leung KW,Chen CL,Wu AT,Lai TC,Li YJ,Hsiao M

Tue Aug 22 00:00:00 UTC 2017

Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.

Acta biochimica et biophysica Sinica

Huang D,Wang S,Wang A,Chen X,Zhang H

Thu Sep 01 00:00:00 UTC 2016

Thymosin beta 4 silencing suppresses proliferation and invasion of non-small cell lung cancer cells by repressing Notch1 activation.

Acta biochimica et biophysica Sinica

Huang D,Wang S,Wang A,Chen X,Zhang H

Thu Sep 01 00:00:00 UTC 2016

Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.

Biochemical and biophysical research communications

Tanaka K,Fujiya M,Konishi H,Ueno N,Kashima S,Sasajima J,Moriichi K,Ikuta K,Tanabe H,Kohgo Y

Fri Nov 20 00:00:00 UTC 2015

Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.

Biochemical and biophysical research communications

Tanaka K,Fujiya M,Konishi H,Ueno N,Kashima S,Sasajima J,Moriichi K,Ikuta K,Tanabe H,Kohgo Y

Fri Nov 20 00:00:00 UTC 2015

Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.

Journal of molecular cell biology

Zhou Y,Li S,Huang Q,Xie L,Zhu X

Thu Aug 01 00:00:00 UTC 2013

Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.

Journal of molecular cell biology

Zhou Y,Li S,Huang Q,Xie L,Zhu X

Thu Aug 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Fx; prothymosin beta 4; prothymosin beta-4; T beta 4; T beta-4; TB4Xbeta 4; thymosin; thymosin beta 4; Thymosin beta-4; thymosin, beta 4, X chromosome

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Gene Aliases: FX; PTMB4; Tb4; TB4X; THYB4; TMSB4; TMSB4X

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UniProt ID: (Human) P62328, (Rat) P62329, (Mouse) P20065

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Entrez Gene ID: (Human) 7114, (Rat) 81814, (Mouse) 19241

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Function(s)

actin monomer binding protein binding poly(A) RNA binding actin binding

Process(es)

osteoblast differentiation actin filament organization regulation of cell migration sequestering of actin monomers positive regulation of smooth muscle cell migration actin cytoskeleton organization positive regulation of transcription, DNA-templated positive regulation of smooth muscle cell differentiation platelet degranulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TMSB4X Polyclonal Antibody

TMSB4X Antibody (19850-1-AP) in IHC (P)

Product Details

19850-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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