Invitrogen

TNFR2 Recombinant Rabbit Monoclonal Antibody (JM113-01)

TNFR2 Antibody in Immunocytochemistry (ICC/IF)

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TNFR2 Antibody (MA5-32618) in ICC/IF

Immunofluorescence analysis of Tumor necrosis factor receptor superfamily member 1B was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TNFR2 Recombinant Rabbit Monoclonal Antibody (JM113-01) (Product # MA5-32618) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cr... View More

TNFR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TNFR2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

TNFR2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

TNFR2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-937 cells were transfected with Tumor necrosis factor receptor superfamily member 1B siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TNFR2 Recombinant Rabbit Monoclonal Antibody (JM113-01) (Product # MA5-32618). {KD}

Product Details

MA5-32618

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:100

Immunoprecipitation (IP)

1:10-1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Pig

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM113-01

Immunogen

Synthetic peptide within Human TNF Receptor II aa 426-461

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2809895

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor necrosis factor receptor-1 is selectively sequestered into Schwann cell extracellular vesicles where it functions as a TNFα decoy.

Glia

Sadri M,Hirosawa N,Le J,Romero H,Martellucci S,Kwon HJ,Pizzo D,Ohtori S,Gonias SL,Campana WM

Published figure using TNFR2 recombinant monoclonal antibody (Product # MA5-32618) in Western Blot

Tue Feb 01 00:00:00 UTC 2022

Upregulated tumor necrosis factor-α transcriptome and proteome in adipose tissue-derived mesenchymal stem cells from pigs with metabolic syndrome.

Cytokine

Pawar AS,Eirin A,Tang H,Zhu XY,Lerman A,Lerman LO

MA5-32618 was used in Immunocytochemistry to investigate whether the inflammatory profile of metabolic syndrome manifests via upregulating MSC mRNAs and proteins of the TNF-α pathway.

Mon Mar 30 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: CD120b; p75; p75 TNF receptor; p75 TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TNF receptor beta chain; TNF-R2; TNF-RII; TNFR; TNFR II; TNFR-II; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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Gene Aliases: CD120b; p75; p75TNFR; TBPII; TNF-alphaR2; TNF-R-II; TNF-R2; TNF-R75; TNFalpha-R2; TNFBR; Tnfr-1; Tnfr-2; TNFR1B; TNFR2; TNFR80; TNFRII; TNFRSF1B

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UniProt ID: (Human) P20333, (Rat) Q80WY6, (Mouse) P25119

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Entrez Gene ID: (Human) 7133, (Rat) 156767, (Mouse) 21938

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Function(s)

tumor necrosis factor-activated receptor activity protein binding ubiquitin protein ligase binding

Process(es)

inflammatory response immune response multicellular organism development aging intrinsic apoptotic signaling pathway in response to DNA damage tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process positive regulation of MAPK cascade negative regulation of inflammatory response RNA destabilization positive regulation of membrane protein ectodomain proteolysis cellular response to lipopolysaccharide cellular response to growth factor stimulus apoptotic signaling pathway extrinsic apoptotic signaling pathway cell surface receptor signaling pathway programmed cell death response to lipopolysaccharide cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TNFR2 Recombinant Rabbit Monoclonal Antibody (JM113-01)

TNFR2 Antibody (MA5-32618) in ICC/IF

Product Details

MA5-32618

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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