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TNKS Polyclonal Antibody

TNKS Antibody in Immunocytochemistry (ICC/IF)

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TNKS Antibody (18030-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using TNKS antibody 18030-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

TNKS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TNKS Antibody in Immunoprecipitation (IP)

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Product Details

18030-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TNKS Fusion Protein Ag12597 (1-329 aa encoded by BC098394)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAASRRSQH HHHHHQQQLQ PAPGASAPPP PPPPPLSPGL APGTTPASPT ASGLAPFASP RHGLALPEGD GSRDPPDRPR SPDQVDGTSC CSTTSTICTV AAAPVVPAVS TSSAAGVAPN PAGSGSNNSP SSSSSPTSSS SSSPSSPGSS LAESPEAAGV SSTAPLGPGA AGPGTGVPAV SGALRELLEA CRNGDVSRVK RLVDAANVNA KDMAGRKSSP LHFAAGFGRK DVVEHLLQMG ANVHARDDGG LIPLHNACSF GHAEVVSLLL CQGADPNARD NWNYTPLHEA AIKGKIDVCI VLLQHGADPN IRNTDGKSAL DLADPSAKAV (1-329 aa encoded by BC098394)

Target Information

Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:10988299, PubMed:11739745, PubMed:16076287, PubMed:19759537, PubMed:21478859, PubMed:22864114, PubMed:23622245, PubMed:25043379). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed:22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed:10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed:16076287). Stimulates 26S proteasome activity (PubMed:23622245). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification and evaluation of a novel PARP1 inhibitor for the treatment of triple-negative breast cancer.

Chemico-biological interactions

Gong R,Ma Z,He L,Jiang S,Cao D,Cheng Y

Fri Sep 01 00:00:00 UTC 2023

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Kim H,Villareal LB,Liu Z,Haneef M,Falcon DM,Martin DR,Lee HJ,Dame MK,Attili D,Chen Y,Varani J,Spence JR,Kovbasnjuk O,Colacino JA,Lyssiotis CA,Lin HC,Shah YM,Xue X

Sat Apr 01 00:00:00 UTC 2023

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Oncology letters

Ma Y,Zhang P,Zhang Q,Wang X,Miao Q,Lyu X,Cui B,Ma H

Fri Oct 01 00:00:00 UTC 2021

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Oncology letters

Ma Y,Zhang P,Zhang Q,Wang X,Miao Q,Lyu X,Cui B,Ma H

Fri Oct 01 00:00:00 UTC 2021

Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.

Oncology letters

Ma Y,Zhang P,Zhang Q,Wang X,Miao Q,Lyu X,Cui B,Ma H

Fri Oct 01 00:00:00 UTC 2021

circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.

Cell death & disease

Ma Z,Han C,Xia W,Wang S,Li X,Fang P,Yin R,Xu L,Yang L

Mon May 11 00:00:00 UTC 2020

circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.

Cell death & disease

Ma Z,Han C,Xia W,Wang S,Li X,Fang P,Yin R,Xu L,Yang L

Mon May 11 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: ADP-ribosyltransferase diphtheria toxin-like 5; ARTD5; HGNC:11941; Poly [ADP-ribose] polymerase 5A; Poly [ADP-ribose] polymerase tankyrase-1; Protein poly-ADP-ribosyltransferase tankyrase-1; TANK1; tankyrase 1; Tankyrase I; tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase; Tankyrase-1; TNKS-1; TRF1-interacting ankyrin-related ADP-ribose polymerase; TRF1-interacting ankyrin-related ADP-ribose polymerase 1

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Gene Aliases: 4930554K12Rik; AI662855; ARTD5; C86528; D130072O21Rik; mTNKS1; PARP-5a; PARP5A; PARPL; pART5; TANK1; TIN1; TINF1; TNKS; TNKS1

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UniProt ID: (Human) O95271, (Mouse) Q6PFX9

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Entrez Gene ID: (Human) 8658, (Mouse) 21951, (Rat) 290794

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Function(s)

NAD+ ADP-ribosyltransferase activity protein binding zinc ion binding histone binding transferase activity transferase activity, transferring glycosyl groups metal ion binding

Process(es)

protein polyubiquitination protein ADP-ribosylation transport cell cycle mitotic nuclear division protein transport Wnt signaling pathway peptidyl-serine phosphorylation peptidyl-threonine phosphorylation positive regulation of telomere maintenance via telomerase negative regulation of DNA binding positive regulation of transcription from RNA polymerase II promoter mRNA transport spindle assembly cell division protein localization to chromosome, telomeric region protein poly-ADP-ribosylation protein auto-ADP-ribosylation positive regulation of canonical Wnt signaling pathway mitotic spindle organization regulation of telomere maintenance via telomerase positive regulation of telomerase activity positive regulation of telomere capping negative regulation of telomere maintenance via telomere lengthening negative regulation of telomeric DNA binding negative regulation of maintenance of mitotic sister chromatid cohesion, telomeric

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TNKS Polyclonal Antibody

TNKS Antibody (18030-1-AP) in ICC/IF

Product Details

18030-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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