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OriGene
TNNT2 Monoclonal Antibody (OTI3H6), TrueMAB™
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FIGURE: 1 / 1
TNNT2 Antibody (CF804472) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNT2 (RC214180, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNT2. Positive lysates LY400130 (100 µg) and LC400130 (20 µg) can be purchased separately from OriGene.
Product Details
CF804472
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI3H6
Immunogen
Full length human recombinant protein of human TNNT2 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Cardiac Troponin T is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Cardiac Troponin T is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. Cardiac Troponin T expression in the human heart is affected by heart failure. Mutations within the Cardiac Troponin T protein have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for the Cardiac Troponin T gene undergo alternative splicing that results in many tissue-specific isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cardiac muscle troponin T; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; cTnT; MGC3889; TnTc; troponin T type 2 (cardiac); Troponin T, cardiac muscle; Troponin T2; troponin T2, cardiac; truncated cardiac troponin T
Gene Aliases: CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TNNT2; TnTC
UniProt ID: (Human) P45379
Entrez Gene ID: (Human) 7139
regulation of heart contraction
muscle filament sliding
negative regulation of ATPase activity
positive regulation of ATPase activity
regulation of muscle filament sliding speed
protein heterooligomerization
response to calcium ion
actin crosslink formation
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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