Proteintech

TNP1 Polyclonal Antibody

TNP1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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TNP1 Antibody (17178-1-AP) in IHC (PFA)

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using (Product # 17178-1-AP) (TNP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

17178-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:200

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TNP1 Fusion Protein Ag10890 (1-55 aa encoded by BC029516)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSTSRKLKS HGMRRSKSRS PHKGVKRGGS KRKYRKGNLK SRKRGDDANR NYRSHL (1-55 aa encoded by BC029516)

Target Information

TNP1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Transition protein-1 is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines (see PRM1, MIM 182880; PRM2, MIM 182890) (Luerssen et al., 1990 ). Sequence Note: removed 4 bases from the 5' end that did not align to the reference genome assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Disrupted intercellular bridges and spermatogenesis in fatty acyl-CoA reductase 1 knockout mice: A new model of ether lipid deficiency.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Pan B,Yuan S,Mayernik L,Yap YT,Moin K,Chung CS,Maddipati K,Krawetz SA,Zhang Z,Hess RA,Chen X

Mon May 01 00:00:00 UTC 2023

Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual.

eLife

Zhu T,Zhang Y,Sheng X,Zhang X,Chen Y,Zhu H,Guo Y,Qi Y,Zhao Y,Zhou Q,Chen X,Guo X,Zhao C

Thu Feb 09 00:00:00 UTC 2023

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Journal of hazardous materials

Peng Z,Yang Q,Yeerken R,Chen J,Liu X,Li X

Tue Feb 15 00:00:00 UTC 2022

Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Journal of hazardous materials

Peng Z,Yang Q,Yeerken R,Chen J,Liu X,Li X

Tue Feb 15 00:00:00 UTC 2022

Cadmium exposure induces pyroptosis in testicular tissue by increasing oxidative stress and activating the AIM2 inflammasome pathway.

The Science of the total environment

Zhou J,Zeng L,Zhang Y,Wang M,Li Y,Jia Y,Wu L,Su P

Tue Nov 15 00:00:00 UTC 2022

CDKN2AIP is critical for spermiogenesis and germ cell development.

Cell & bioscience

Cao Y,Sun Q,Chen Z,Lu J,Geng T,Ma L,Zhang Y

Sun Aug 21 00:00:00 UTC 2022

CDKN2AIP is critical for spermiogenesis and germ cell development.

Cell & bioscience

Cao Y,Sun Q,Chen Z,Lu J,Geng T,Ma L,Zhang Y

Sun Aug 21 00:00:00 UTC 2022

Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.

Environmental pollution (Barking, Essex : 1987)

Liu J,Li X,Zhou G,Zhang Y,Sang Y,Wang J,Li Y,Ge W,Sun Z,Zhou X

Wed Sep 01 00:00:00 UTC 2021

CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation.

Zygote (Cambridge, England)

Yu Y,Wang J,Zhou L,Li H,Zheng B,Yang S

Mon Feb 01 00:00:00 UTC 2021

Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†.

Biology of reproduction

Devlin DJ,Nozawa K,Ikawa M,Matzuk MM

Tue Aug 04 00:00:00 UTC 2020

PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis.

Cell reports

Kim CR,Noda T,Kim H,Kim G,Park S,Na Y,Oura S,Shimada K,Bang I,Ahn JY,Kim YR,Oh SK,Choi HJ,Kim JS,Jung I,Lee H,Okada Y,Ikawa M,Baek SH

Tue Jul 28 00:00:00 UTC 2020

Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice.

Environmental pollution (Barking, Essex : 1987)

Liu J,Li X,Zhou G,Sang Y,Zhang Y,Zhao Y,Ge W,Sun Z,Zhou X

Thu Oct 01 00:00:00 UTC 2020

The ubiquitin ligase subunit β-TrCP in Sertoli cells is essential for spermatogenesis in mice.

Developmental biology

Morohoshi A,Nakagawa T,Nakano S,Nagasawa Y,Nakayama K

Tue Jan 15 00:00:00 UTC 2019

The ubiquitin ligase subunit β-TrCP in Sertoli cells is essential for spermatogenesis in mice.

Developmental biology

Morohoshi A,Nakagawa T,Nakano S,Nagasawa Y,Nakayama K

Tue Jan 15 00:00:00 UTC 2019

PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.

Development (Cambridge, England)

Wang X,Kang JY,Wei L,Yang X,Sun H,Yang S,Lu L,Yan M,Bai M,Chen Y,Long J,Li N,Li D,Huang J,Lei M,Shao Z,Yuan W,Zuo E,Lu K,Liu MF,Li J

Wed Jul 10 00:00:00 UTC 2019

Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.

Biology of reproduction

Xiong Z,Wang C,Wang Z,Dai H,Song Q,Zou Z,Xiao B,Zhao AZ,Bai X,Chen Z

Sat Dec 01 00:00:00 UTC 2018

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.

Cell

Gou LT,Kang JY,Dai P,Wang X,Li F,Zhao S,Zhang M,Hua MM,Lu Y,Zhu Y,Li Z,Chen H,Wu LG,Li D,Fu XD,Li J,Shi HJ,Liu MF

Thu Jun 01 00:00:00 UTC 2017

Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.

Cell

Gou LT,Kang JY,Dai P,Wang X,Li F,Zhao S,Zhang M,Hua MM,Lu Y,Zhu Y,Li Z,Chen H,Wu LG,Li D,Fu XD,Li J,Shi HJ,Liu MF

Thu Jun 01 00:00:00 UTC 2017

Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis.

Molecular cell

Liu S,Yu H,Liu Y,Liu X,Zhang Y,Bu C,Yuan S,Chen Z,Xie G,Li W,Xu B,Yang J,He L,Jin T,Xiong Y,Sun L,Liu X,Han C,Cheng Z,Liang J,Shang Y

Thu Sep 07 00:00:00 UTC 2017

Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis.

Molecular cell

Liu S,Yu H,Liu Y,Liu X,Zhang Y,Bu C,Yuan S,Chen Z,Xie G,Li W,Xu B,Yang J,He L,Jin T,Xiong Y,Sun L,Liu X,Han C,Cheng Z,Liang J,Shang Y

Thu Sep 07 00:00:00 UTC 2017

TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis.

Journal of cell science

Jha KN,Tripurani SK,Johnson GR

Mon May 15 00:00:00 UTC 2017

TSSK6 is required for γH2AX formation and the histone-to-protamine transition during spermiogenesis.

Journal of cell science

Jha KN,Tripurani SK,Johnson GR

Mon May 15 00:00:00 UTC 2017

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Reproductive sciences (Thousand Oaks, Calif.)

Esakky P,Hansen DA,Drury AM,Moley KH

Fri Mar 01 00:00:00 UTC 2013

Molecular analysis of cell type-specific gene expression profile during mouse spermatogenesis by laser microdissection and qRT-PCR.

Reproductive sciences (Thousand Oaks, Calif.)

Esakky P,Hansen DA,Drury AM,Moley KH

Fri Mar 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: Spermatid nuclear transition protein 1; STP-1; Testis-specific basic protein; tnp; TP-1; transition protein 1 (during histone to protamine replacement)

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Gene Aliases: Stp-1; TNP1; Tp-1; TP1

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UniProt ID: (Human) P09430, (Rat) P02317, (Mouse) P10856

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Entrez Gene ID: (Human) 7141, (Rat) 24839, (Mouse) 21958

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Function(s)

DNA binding protein binding

Process(es)

single strand break repair nucleosome disassembly chromatin remodeling chromatin silencing multicellular organism development spermatogenesis spermatid development spermatid nucleus elongation sexual reproduction sperm motility negative regulation of transcription, DNA-templated spermatid nucleus differentiation fertilization, exchange of chromosomal proteins cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TNP1 Polyclonal Antibody

TNP1 Antibody (17178-1-AP) in IHC (PFA)

Product Details

17178-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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