Proteintech

TOB1 Polyclonal Antibody

TOB1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

TOB1 Antibody (14915-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 14915-1-AP (TOB1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TOB1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TOB1 Antibody in Immunoprecipitation (IP)

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Product Details

14915-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TOB1 Fusion Protein Ag6714 (1-345 aa encoded by BC031406)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

14915-1-AP recognizes 38 and 45 kDa bands. The additional 45 kDa band may be due to phosphorylation.

Immunogen sequence: MQLEIQVAL NFIISYLYNK LPRRRVNIFG EELERLLKKK YEGHWYPEKP YKGSGFRCIH IGEKVDPVIE QASKESGLDI DDVRGNLPQD LSVWIDPFEV SYQIGEKGPV KVLYVDDNNE NGCELDKEIK NSFNPEAQVF MPISDPASSV SSSPSPPFGH SAAVSPTFMP RSTQPLTFTT ATFAATKFGS TKMKNSGRSN KVARTSPINL GLNVNDLLKQ KAISSSMHSL YGLGLGSQQQ PQQQQQPAQP PPPPPPPQQQ QQQKTSALSP NAKEFIFPNM QGQGSSTNGM FPGDSPLNLS PLQYSNAFDV FAAYGGLNEK SFVDGLNFSL NNMQYSNQQF QPVMAN (1-345 aa encoded by B C031406)

Target Information

This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effect of mir-92a-3p on hydroquinone induced changes in human lymphoblastoid cell cycle and apoptosis.

Environmental toxicology

Xuan M,Wu Y,Wang H,Ye Z,Wu H,Chen Y,Yang H,Tang H

Thu Jun 01 00:00:00 UTC 2023

Control of immediate early gene expression by CPEB4-repressor complex-mediated mRNA degradation.

Genome biology

Poetz F,Lebedeva S,Schott J,Lindner D,Ohler U,Stoecklin G

Mon Sep 12 00:00:00 UTC 2022

Preferential translation of p53 target genes.

RNA biology

Hisaoka M,Schott J,Bortecen T,Lindner D,Krijgsveld J,Stoecklin G

Fri Apr 08 00:00:00 UTC 2022

TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression.

Communications biology