Proteintech

TOP1 Polyclonal Antibody

TOP1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

TOP1 Antibody (20705-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 20705-1-AP (TOP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

TOP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

20705-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to TOP1.

Target Information

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Anti-tumor effects of P-LPK-CPT, a peptide-camptothecin conjugate, in colorectal cancer.

Communications biology

Hou L,Hou Y,Liang Y,Chen B,Zhang X,Wang Y,Zhou K,Zhong T,Long B,Pang W,Wang L,Han X,Li L,Xu C,Gross I,Gaiddon C,Fu W,Yao H,Meng X

Mon Nov 14 00:00:00 UTC 2022

HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer.

Cell death & disease

Ma H,Qi G,Han F,Lu W,Peng J,Li R,Yan S,Yuan C,Kong B

Thu Mar 24 00:00:00 UTC 2022

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Cell chemical biology

Flor A,Wolfgeher D,Li J,Hanakahi LA,Kron SJ

Thu Jun 17 00:00:00 UTC 2021

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Cell chemical biology

Flor A,Wolfgeher D,Li J,Hanakahi LA,Kron SJ

Thu Jun 17 00:00:00 UTC 2021

Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.

iScience

Hao X,Zhao B,Zhou W,Liu H,Fukumoto T,Gabrilovich D,Zhang R

Fri Jan 22 00:00:00 UTC 2021

EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.

Journal of cellular and molecular medicine

Wu W,Dong J,Gou H,Geng R,Yang X,Chen D,Xiang B,Zhang Z,Ren S,Chen L,Liu J

Sun Aug 01 00:00:00 UTC 2021

Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling.

Nature aging

Cai D,Han JJ

Sat May 01 00:00:00 UTC 2021

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.

Nature communications

Zhao B,Liu P,Fukumoto T,Nacarelli T,Fatkhutdinov N,Wu S,Lin J,Aird KM,Tang HY,Liu Q,Speicher DW,Zhang R

Wed Feb 19 00:00:00 UTC 2020

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.

Nature communications

Zhao B,Liu P,Fukumoto T,Nacarelli T,Fatkhutdinov N,Wu S,Lin J,Aird KM,Tang HY,Liu Q,Speicher DW,Zhang R

Wed Feb 19 00:00:00 UTC 2020

Transcription modulation by CDK9 regulates inflammatory genes and RIPK3-MLKL-mediated necroptosis in periodontitis progression.

Scientific reports

Li J,Shi J,Pan Y,Zhao Y,Yan F,Li H,Lei L

Fri Nov 22 00:00:00 UTC 2019

IFN-γ restores the impaired function of RNase L and induces mitochondria-mediated apoptosis in lung cancer.

Cell death & disease

Yin H,Jiang Z,Wang S,Zhang P

Mon Sep 09 00:00:00 UTC 2019

Treatment mechanism of matrine in combination with irinotecan for colon cancer.

Oncology letters

Duan L,Deng L,Wang D,Ma S,Li C,Zhao D

Tue Aug 01 00:00:00 UTC 2017

Treatment mechanism of matrine in combination with irinotecan for colon cancer.

Oncology letters

Duan L,Deng L,Wang D,Ma S,Li C,Zhao D

Tue Aug 01 00:00:00 UTC 2017

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Chemico-biological interactions

Wu J,Yang C,Guo C,Li X,Yang N,Zhao L,Hang H,Liu S,Chu P,Sun Z,Sun B,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Jan 25 00:00:00 UTC 2016

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Chemico-biological interactions

Wu J,Yang C,Guo C,Li X,Yang N,Zhao L,Hang H,Liu S,Chu P,Sun Z,Sun B,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Jan 25 00:00:00 UTC 2016

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Oncology reports

Gao L,Xu Z,Wang Y,Sun B,Song Z,Yang B,Liu X,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Feb 01 00:00:00 UTC 2016

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Oncology reports

Gao L,Xu Z,Wang Y,Sun B,Song Z,Yang B,Liu X,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Feb 01 00:00:00 UTC 2016

Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.

Oncotarget

Lingzi X,Zhihua Y,Xuelian L,Yangwu R,Haibo Z,Yuxia Z,Baosen Z

Tue Dec 13 00:00:00 UTC 2016

Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.

Oncotarget

Lingzi X,Zhihua Y,Xuelian L,Yangwu R,Haibo Z,Yuxia Z,Baosen Z

Tue Dec 13 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: DNA topoisomerase 1; DNA topoisomerase I; RP3-511B24.2; type I DNA topoisomerase

View more View less

Gene Aliases: TOP1; TOPI

View more View less

UniProt ID: (Human) P11387

View more View less

Entrez Gene ID: (Human) 7150

View more View less

Function(s)

core promoter sequence-specific DNA binding DNA binding chromatin binding DNA topoisomerase type I activity DNA topoisomerase type II (ATP-hydrolyzing) activity protein binding poly(A) RNA binding

Process(es)

DNA replication DNA topological change chromatin remodeling chromosome segregation circadian rhythm programmed cell death viral process phosphorylation protein sumoylation circadian regulation of gene expression embryonic cleavage response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

TOP1 Polyclonal Antibody

TOP1 Antibody (20705-1-AP) in ICC/IF

Product Details

20705-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!