Proteintech

TOP2A Polyclonal Antibody

TOP2A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

TOP2A Antibody (20233-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using TOP2A antibody 20233-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

TOP2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TOP2A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20233-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to the gene TOP2A.

Target Information

Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Magnetic Fields Affect Alcoholic Liver Disease by Liver Cell Oxidative Stress and Proliferation Regulation.

Research (Washington, D.C.)

Song C,Chen H,Yu B,Zhang L,Wang J,Feng C,Yang X,Tian X,Fan Y,Ji X,Wang H,Xie C,Zhang X

Wed Apr 05 00:00:00 UTC 2023

Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.

Nature communications

Luo Z,Xin D,Liao Y,Berry K,Ogurek S,Zhang F,Zhang L,Zhao C,Rao R,Dong X,Li H,Yu J,Lin Y,Huang G,Xu L,Xin M,Nishinakamura R,Yu J,Kool M,Pfister SM,Roussel MF,Zhou W,Weiss WA,Andreassen P,Lu QR

Fri Feb 10 00:00:00 UTC 2023

Chalcone Derivative CX258 Suppresses Colorectal Cancer via Inhibiting the TOP2A/Wnt/β-Catenin Signaling.

Cells

Chen X,Lv X,Gao L,Liu J,Wang W,Guo L,Frasinyuk MS,Zhang W,Watt DS,Liu C,Liu X

Fri Mar 31 00:00:00 UTC 2023

Optimization of the Natural Product Calothrixin A to Discover Novel Dual Topoisomerase I and II Inhibitors with Improved Anticancer Activity.

Journal of medicinal chemistry

Yang X,Wang ZP,Xiang S,Wang D,Zhao Y,Luo D,Qiu Y,Huang C,Guo J,Dai Y,Zhang SL,He Y

Thu Jun 09 00:00:00 UTC 2022

The mechanism of acentrosomal spindle assembly in human oocytes.

Science (New York, N.Y.)

Wu T,Dong J,Fu J,Kuang Y,Chen B,Gu H,Luo Y,Gu R,Zhang M,Li W,Dong X,Sun X,Sang Q,Wang L

Fri Nov 18 00:00:00 UTC 2022

LRR1-mediated replisome disassembly promotes DNA replication by recycling replisome components.

The Journal of cell biology

Fan Y,Köberlin MS,Ratnayeke N,Liu C,Deshpande M,Gerhardt J,Meyer T

Mon Aug 02 00:00:00 UTC 2021

Small Nucleolar RNAs Determine Resistance to Doxorubicin in Human Osteosarcoma.

International journal of molecular sciences

Godel M,Morena D,Ananthanarayanan P,Buondonno I,Ferrero G,Hattinger CM,Di Nicolantonio F,Serra M,Taulli R,Cordero F,Riganti C,Kopecka J

Wed Jun 24 00:00:00 UTC 2020

Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.

Cancer treatment and research communications

Cattrini C,Capaia M,Boccardo F,Barboro P

Wed Nov 17 00:00:00 UTC 2021

Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2α and miR-494.

BioMed research international

Fang Y,Yang C,Zhang L,Wei L,Lin J,Zhao J,Peng J

Thu Mar 19 00:00:00 UTC 2020

Identifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis.

Journal of Cancer

Xiong Y,Yuan L,Chen L,Zhu Y,Zhang S,Liu X,Xiao Y,Wang X

Wed Sep 30 00:00:00 UTC 2020

Identifying a Novel Biomarker TOP2A of Clear Cell Renal Cell Carcinoma (ccRCC) Associated with Smoking by Co-Expression Network Analysis.

Journal of Cancer

Xiong Y,Yuan L,Chen L,Zhu Y,Zhang S,Liu X,Xiao Y,Wang X

Wed Sep 30 00:00:00 UTC 2020

3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

Journal of experimental & clinical cancer research : CR

Li W,Liu J,Fu W,Zheng X,Ren L,Liu S,Wang J,Ji T,Du G

Tue Jul 03 00:00:00 UTC 2018

3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.

Journal of experimental & clinical cancer research : CR

Li W,Liu J,Fu W,Zheng X,Ren L,Liu S,Wang J,Ji T,Du G

Tue Jul 03 00:00:00 UTC 2018

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Oncology reports

Gao L,Xu Z,Wang Y,Sun B,Song Z,Yang B,Liu X,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Feb 01 00:00:00 UTC 2016

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Oncology reports

Gao L,Xu Z,Wang Y,Sun B,Song Z,Yang B,Liu X,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Feb 01 00:00:00 UTC 2016

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

Science (New York, N.Y.)

Minajigi A,Froberg J,Wei C,Sunwoo H,Kesner B,Colognori D,Lessing D,Payer B,Boukhali M,Haas W,Lee JT

Fri Jul 17 00:00:00 UTC 2015

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

Science (New York, N.Y.)

Minajigi A,Froberg J,Wei C,Sunwoo H,Kesner B,Colognori D,Lessing D,Payer B,Boukhali M,Haas W,Lee JT

Fri Jul 17 00:00:00 UTC 2015

High glucose promotes gastric cancer chemoresistance in vivo and in vitro.

Molecular medicine reports

Zhao W,Chen R,Zhao M,Li L,Fan L,Che XM

Wed Jul 01 00:00:00 UTC 2015

High glucose promotes gastric cancer chemoresistance in vivo and in vitro.

Molecular medicine reports

Zhao W,Chen R,Zhao M,Li L,Fan L,Che XM

Wed Jul 01 00:00:00 UTC 2015

Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.

Toxicological sciences : an official journal of the Society of Toxicology

Lu C,Liu X,Liu C,Wang J,Li C,Liu Q,Li Y,Li S,Sun S,Yan J,Shao J

Thu Oct 01 00:00:00 UTC 2015

Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.

Toxicological sciences : an official journal of the Society of Toxicology

Lu C,Liu X,Liu C,Wang J,Li C,Liu Q,Li Y,Li S,Sun S,Yan J,Shao J

Thu Oct 01 00:00:00 UTC 2015

TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.

Oncotarget

Li X,Liu Y,Chen W,Fang Y,Xu H,Zhu HH,Chu M,Li W,Zhuang G,Gao WQ

Wed Oct 15 00:00:00 UTC 2014

TOP2Ahigh is the phenotype of recurrence and metastasis whereas TOP2Aneg cells represent cancer stem cells in prostate cancer.

Oncotarget

Li X,Liu Y,Chen W,Fang Y,Xu H,Zhu HH,Chu M,Li W,Zhuang G,Gao WQ

Wed Oct 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha

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Gene Aliases: TOP2; TOP2A; TP2A

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UniProt ID: (Human) P11388

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Entrez Gene ID: (Human) 7153

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Function(s)

magnesium ion binding DNA binding chromatin binding DNA topoisomerase type II (ATP-hydrolyzing) activity protein kinase C binding protein binding ATP binding protein C-terminus binding DNA-dependent ATPase activity drug binding DNA binding, bending enzyme binding protein homodimerization activity histone deacetylase binding ubiquitin binding poly(A) RNA binding protein heterodimerization activity

Process(es)

resolution of meiotic recombination intermediates sister chromatid segregation hematopoietic progenitor cell differentiation DNA topological change DNA ligation DNA unwinding involved in DNA replication mitotic recombination cellular response to DNA damage stimulus chromosome segregation protein sumoylation apoptotic chromosome condensation embryonic cleavage regulation of circadian rhythm positive regulation of apoptotic process mitotic DNA integrity checkpoint positive regulation of viral genome replication positive regulation of single stranded viral RNA replication via double stranded DNA intermediate positive regulation of transcription from RNA polymerase II promoter rhythmic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TOP2A Polyclonal Antibody

TOP2A Antibody (20233-1-AP) in ICC/IF

Product Details

20233-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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