Proteintech

TOP2A Polyclonal Antibody

TOP2A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

TOP2A Antibody (24641-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 24641-1-AP (TOP2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

TOP2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TOP2A Antibody in Immunoprecipitation (IP)

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Product Details

24641-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

TOP2A Fusion Protein Ag19551 (1187-1531 aa encoded by BC140791)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.53 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QDEQVGLPG KGGKAKGKKT QMAEVLPSPR GQRVIPRITI EMKAEAEKKN KKKIKNENTE GSPQEDGVEL EGLKQRLEKK QKREPGTKTK KQTTLAFKPI KKGKKRNPWS DSESDRSSDE SNFDVPPRET EPRRAATKTK FTMDLDSDED FSDFDEKTDD EDFVPSDASP PKTKTSPKLS NKELKPQKSV VSDLEADDVK GSVPLSSSPP ATHFPDETEI TNPVPKKNVT VKKTAAKSQS STSTTGAKKR AAPKGTKRDP ALNSGVSQKP DPAKTKNRRK RKPSTSDDSD SNFEKIVSKA VTSKKSKGES DDFHMDFDSA VAPRAKSVRA KKPIKYLEES DEDDLF (1187-1531 aa encoded by BC140791)

Target Information

Topo II alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2 and M-phases in transformed and developmentally regulated normal cellsTopo II alpha is also implicated in drug resistance of tumor cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Kinase activity profiling identifies putative downstream targets of cGMP/PKG signaling in inherited retinal neurodegeneration.

Cell death discovery

Roy A,Tolone A,Hilhorst R,Groten J,Tomar T,Paquet-Durand F

Thu Mar 03 00:00:00 UTC 2022

Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC.

Frontiers in cell and developmental biology

Chen MY,Zeng YC,Zhao XH

Sat Jul 16 00:00:00 UTC 2022

High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia

Zhou X,Niu X,Mao Q,Liu Y

Sat May 01 00:00:00 UTC 2021

SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.

Molecular biology of the cell

Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

Sun Mar 15 00:00:00 UTC 2020

SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.

Molecular biology of the cell

Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

Sun Mar 15 00:00:00 UTC 2020

SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.

Molecular biology of the cell

Watanabe R,Maekawa M,Hieda M,Taguchi T,Miura N,Kikugawa T,Saika T,Higashiyama S

Sun Mar 15 00:00:00 UTC 2020

Novel gene-specific translation mechanism of dysregulated, chronic inflammation reveals promising, multifaceted COVID-19 therapeutics.

bioRxiv : the preprint server for biology

Wang L,Muneer A,Xie L,Zhang F,Wu B,Mei L,Lenarcic EM,Feng EH,Song J,Xiong Y,Yu X,Wang C,Gheorghe C,Torralba K,Cook JG,Wan YY,Moorman NJ,Song H,Jin J,Chen X

Mon Nov 16 00:00:00 UTC 2020

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Chemico-biological interactions

Wu J,Yang C,Guo C,Li X,Yang N,Zhao L,Hang H,Liu S,Chu P,Sun Z,Sun B,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Jan 25 00:00:00 UTC 2016

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Chemico-biological interactions

Wu J,Yang C,Guo C,Li X,Yang N,Zhao L,Hang H,Liu S,Chu P,Sun Z,Sun B,Lin Y,Peng J,Han G,Wang S,Tang Z

Mon Jan 25 00:00:00 UTC 2016

Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.

Developmental biology

Martin JH,Nixon B,Lord T,Bromfield EG,Aitken RJ

Thu Sep 01 00:00:00 UTC 2016

Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.

Developmental biology

Martin JH,Nixon B,Lord T,Bromfield EG,Aitken RJ

Thu Sep 01 00:00:00 UTC 2016

Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: a proteomic study.

Oncology reports

Liu JS,He SC,Zhang ZL,Chen R,Fan L,Qiu GL,Chang S,Li L,Che XM

Mon Dec 01 00:00:00 UTC 2014

Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: a proteomic study.

Oncology reports

Liu JS,He SC,Zhang ZL,Chen R,Fan L,Qiu GL,Chang S,Li L,Che XM

Mon Dec 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA Topoisomerase II alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; EC 5.99.1.3; OTTHUMP00000207108; P11388-1; topoisomerase (DNA) II alpha 170kDa; topoisomerase ii alpha

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Gene Aliases: Top-2; TOP2; TOP2A; TP2A

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UniProt ID: (Human) P11388, (Mouse) Q01320

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Entrez Gene ID: (Human) 7153, (Mouse) 21973

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Function(s)

magnesium ion binding DNA binding chromatin binding DNA topoisomerase type II (ATP-hydrolyzing) activity protein kinase C binding protein binding ATP binding protein C-terminus binding DNA-dependent ATPase activity drug binding DNA binding, bending enzyme binding protein homodimerization activity histone deacetylase binding ubiquitin binding poly(A) RNA binding protein heterodimerization activity nucleotide binding DNA topoisomerase activity isomerase activity sequence-specific DNA binding structure-specific DNA binding metal ion binding

Process(es)

resolution of meiotic recombination intermediates sister chromatid segregation hematopoietic progenitor cell differentiation DNA topological change DNA ligation DNA unwinding involved in DNA replication mitotic recombination cellular response to DNA damage stimulus chromosome segregation protein sumoylation apoptotic chromosome condensation embryonic cleavage regulation of circadian rhythm positive regulation of apoptotic process mitotic DNA integrity checkpoint positive regulation of viral genome replication positive regulation of single stranded viral RNA replication via double stranded DNA intermediate positive regulation of transcription from RNA polymerase II promoter rhythmic process DNA metabolic process chromosome condensation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TOP2A Polyclonal Antibody

TOP2A Antibody (24641-1-AP) in ICC/IF

Product Details

24641-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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