Proteintech

TOP2B Polyclonal Antibody

TOP2B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

TOP2B Antibody (20549-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

TOP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TOP2B Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

20549-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to TOP2B.

Target Information

TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Short-Term Starvation Weakens the Efficacy of Cell Cycle Specific Chemotherapy Drugs through G1 Arrest.

International journal of molecular sciences

Shi M,Hou J,Shao S,Liang W,Wang S,Yang Y,Guo Z,Pan F

Sat Jan 28 00:00:00 UTC 2023

MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".

Molecular cell

Das SK,Kuzin V,Cameron DP,Sanford S,Jha RK,Nie Z,Rosello MT,Holewinski R,Andresson T,Wisniewski J,Natsume T,Price DH,Lewis BA,Kouzine F,Levens D,Baranello L

Thu Jan 06 00:00:00 UTC 2022

MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".

Molecular cell

Das SK,Kuzin V,Cameron DP,Sanford S,Jha RK,Nie Z,Rosello MT,Holewinski R,Andresson T,Wisniewski J,Natsume T,Price DH,Lewis BA,Kouzine F,Levens D,Baranello L

Thu Jan 06 00:00:00 UTC 2022

DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells.

RNA (New York, N.Y.)

David M,Olender T,Mizrahi O,Weingarten-Gabbay S,Friedlander G,Meril S,Goldberg N,Savidor A,Levin Y,Salomon V,Stern-Ginossar N,Bialik S,Kimchi A

Sat Oct 01 00:00:00 UTC 2022

Aconitine linoleate, a natural lipo-diterpenoid alkaloid, stimulates anti-proliferative activity reversing doxorubicin resistance in MCF-7/ADR breast cancer cells as a selective topoisomerase IIα inhibitor.

Naunyn-Schmiedeberg's archives of pharmacology

Luan S,Gao Y,Liang X,Zhang L,Wu Q,Hu Y,Yin L,He C,Liu S

Sat Jan 01 00:00:00 UTC 2022

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Journal of medicinal chemistry

Zhou Z,Li Y,Ma X,Cao B,Peng T,Sheng Y,Peng H,Li R,Cao Y,Xi R,Li F,Wang M,Sun H,Zhang G,Zhang H,Hu K,Xiao W,Wang F

Thu Jun 10 00:00:00 UTC 2021

Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.

Cell reports

Herrero-Ruiz A,Martínez-García PM,Terrón-Bautista J,Millán-Zambrano G,Lieberman JA,Jimeno-González S,Cortés-Ledesma F

Tue Apr 13 00:00:00 UTC 2021

Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.

Cell reports

Herrero-Ruiz A,Martínez-García PM,Terrón-Bautista J,Millán-Zambrano G,Lieberman JA,Jimeno-González S,Cortés-Ledesma F

Tue Apr 13 00:00:00 UTC 2021

Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.

Cancer treatment and research communications

Cattrini C,Capaia M,Boccardo F,Barboro P

Wed Nov 17 00:00:00 UTC 2021

Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.

Scientific reports

Sun LY,Zhu LW,Tang YJ

Mon Oct 08 00:00:00 UTC 2018

Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.

Scientific reports

Sun LY,Zhu LW,Tang YJ

Mon Oct 08 00:00:00 UTC 2018

Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.

Scientific reports

Xu F,Fang Y,Yan L,Xu L,Zhang S,Cao Y,Xu L,Zhang X,Xie J,Jiang G,Ge C,An N,Zhou D,Yuan N,Wang J

Mon Mar 27 00:00:00 UTC 2017

Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.

Scientific reports

Xu F,Fang Y,Yan L,Xu L,Zhang S,Cao Y,Xu L,Zhang X,Xie J,Jiang G,Ge C,An N,Zhou D,Yuan N,Wang J

Mon Mar 27 00:00:00 UTC 2017

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

Science (New York, N.Y.)

Minajigi A,Froberg J,Wei C,Sunwoo H,Kesner B,Colognori D,Lessing D,Payer B,Boukhali M,Haas W,Lee JT

Fri Jul 17 00:00:00 UTC 2015

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

Science (New York, N.Y.)

Minajigi A,Froberg J,Wei C,Sunwoo H,Kesner B,Colognori D,Lessing D,Payer B,Boukhali M,Haas W,Lee JT

Fri Jul 17 00:00:00 UTC 2015

Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.

Toxicological sciences : an official journal of the Society of Toxicology

Lu C,Liu X,Liu C,Wang J,Li C,Liu Q,Li Y,Li S,Sun S,Yan J,Shao J

Thu Oct 01 00:00:00 UTC 2015

Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.

Toxicological sciences : an official journal of the Society of Toxicology

Lu C,Liu X,Liu C,Wang J,Li C,Liu Q,Li Y,Li S,Sun S,Yan J,Shao J

Thu Oct 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen

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Gene Aliases: D230016L12Rik; Top-2; TOP2B; top2beta; TOPIIB

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UniProt ID: (Human) Q02880, (Mouse) Q64511

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Entrez Gene ID: (Human) 7155, (Mouse) 21974

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Function(s)

chromatin binding DNA topoisomerase type II (ATP-hydrolyzing) activity protein kinase C binding ATP binding protein C-terminus binding DNA binding, bending enzyme binding histone deacetylase binding metal ion binding protein heterodimerization activity nucleotide binding DNA binding DNA topoisomerase activity protein binding isomerase activity

Process(es)

resolution of meiotic recombination intermediates sister chromatid segregation neuron migration DNA topological change DNA unwinding involved in DNA replication mitotic recombination axonogenesis protein sumoylation forebrain development mitotic DNA integrity checkpoint DNA metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TOP2B Polyclonal Antibody

TOP2B Antibody (20549-1-AP) in ICC/IF

Product Details

20549-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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