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Invitrogen
TOP2B Monoclonal Antibody (681417)
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FIGURE: 1 / 4
TOP2B Antibody (MA5-24310) in ICC/IF
Immunocytochemical analysis of TOP2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using mouse Anti-human/mouse TOP2B Monoclonal Antibody (Product # MA5-24310) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-mouse IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Product Details
MA5-24310
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
681417
Immunogen
E. coli-derived recombinant human TOP2B Phe1192-Asp1621
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608145
Product Specific Information
In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse TOP2B and no cross-reactivity with recombinant human TOP2A is observed.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen
Gene Aliases: D230016L12Rik; Top-2; TOP2B; top2beta; TOPIIB
UniProt ID: (Human) Q02880, (Mouse) Q64511
Entrez Gene ID: (Human) 7155, (Mouse) 21974
chromatin binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
ATP binding
protein C-terminus binding
DNA binding, bending
enzyme binding
histone deacetylase binding
metal ion binding
protein heterodimerization activity
nucleotide binding
DNA binding
DNA topoisomerase activity
protein binding
isomerase activity
Performance Guarantee
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