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Invitrogen
TOP3A Polyclonal Antibody
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FIGURE: 1 / 2
TOP3A Antibody (PA5-101160) in ICC/IF
Immunofluorescent analysis of TOP3A in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and TOP3A polyclonal antibody (Product # PA5-101160) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-101160
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TOP3A(Accession Q13472), corresponding to amino acid residues D953-R1001.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815882
Product Specific Information
Antibody detects endogenous levels of total TOP3A.
Target Information
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIalpha exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIalpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo IIIbeta exists as three isoforms,beta-1,beta -2 and beta-3, also produced by alternative splicing. Topo IIIbeta-1 is expressed in testis, heart, and skeletal muscle, whereas Topo IIIbeta-2 is expressed in thymus, kidney and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; Top IIIa; topo III-alpha; topoisomerase (DNA) 3 alpha; topoisomerase 3 alpha; zinc finger, GRF-type containing 7
Gene Aliases: TOP3; TOP3A; ZGRF7
UniProt ID: (Human) Q13472, (Mouse) O70157
Entrez Gene ID: (Human) 7156, (Mouse) 21975
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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