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TOP3A Antibody (PA5-121538) in IHC (P)
Immunohistochemistry of TOP3A in paraffin-embedded human prostate cancer tissue. Samples were incubated in polyclonal TOP3A antibody (Product # PA5-121538) using a dilution of 20 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG at a dilution of 2 µg/mL and DAB.
Product Details
PA5-121538
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Lys35-Ser179 (Accession Q13472), with N-terminal His Tag
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2915110
Target Information
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIalpha exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIalpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo IIIbeta exists as three isoforms,beta-1,beta -2 and beta-3, also produced by alternative splicing. Topo IIIbeta-1 is expressed in testis, heart, and skeletal muscle, whereas Topo IIIbeta-2 is expressed in thymus, kidney and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; topo III-alpha; zinc finger, GRF-type containing 7
Gene Aliases: TOP3; TOP3A; ZGRF7
UniProt ID: (Human) Q13472
Entrez Gene ID: (Human) 7156
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