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Invitrogen
TOPORS Polyclonal Antibody
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FIGURE: 1 / 1
TOPORS Antibody (PA5-116849) in WB
Western blot analysis of TOPORS in extracts of various cell lines. Samples were incubated with TOPORS Polyclonal antibody (Product # PA5-116849) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
PA5-116849
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse Topors (NP_5988582)
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901480
Product Specific Information
Positive Samples: Mouse liver, Mouse lung, Mouse kideny, Rat kidney
Immunogen sequence: PDSKCPICLD RFDNVSYLDR CLHKFCFRCV QEWSKNKAEC PLCKQPFDSI FHSVRAEDDF KEYVLRPSYN GSFTNPEVRR FRYRTTMTRE RSASLYSPSS T
Target Information
Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage-induced cell death through IKBKE sumoylation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase Topors; p53-binding protein 3; p53BP3; RING-type E3 ubiquitin transferase Topors; SUMO1-protein E3 ligase Topors; topoisomerase 1-binding RING finger; Topoisomerase I-binding arginine/serine-rich protein; Topoisomerase I-binding RING finger protein; Tumor suppressor p53-binding protein 3
Gene Aliases: AW105885; LUN; p53BP3/LUN; Topors; TP53BPL
UniProt ID: (Mouse) Q80Z37
Entrez Gene ID: (Mouse) 106021, (Rat) 362501
DNA binding
antigen binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
SUMO transferase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
DNA topoisomerase binding
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
isomerase activity
ubiquitin-dependent protein catabolic process
protein monoubiquitination
cellular response to DNA damage stimulus
intrinsic apoptotic signaling pathway in response to DNA damage
protein sumoylation
protein localization to nucleus
regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of transcription, DNA-templated
positive regulation of ubiquitin-protein transferase activity
maintenance of protein location in nucleus
protein K48-linked ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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