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TOX Antibody (PA5-30328) in IHC (P)
TOX Polyclonal Antibody detects TOX protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. TOX stained by TOX Polyclonal Antibody (Product # PA5-30328) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Product Details
PA5-30328
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human TOX. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2547802
Product Specific Information
Recommended positive controls: THP-1, HL-60.
Predicted reactivity: Mouse (89%), Rat (91%), Rhesus Monkey (99%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; Thymus high mobility group box protein TOX
Gene Aliases: 1700007F02Rik; KIAA0808; TOX; TOX1
UniProt ID: (Human) O94900, (Mouse) Q66JW3
Entrez Gene ID: (Human) 9760, (Mouse) 252838
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