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Invitrogen
TPH1 Polyclonal Antibody
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Product Details
PA1-4678
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit,
Rat
Published species
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_561855
Product Specific Information
This antibody is predicted to react with wide variety of mammals based on 100% sequence homology, however it does not recognize TPH in rabbit tissues.
This antibody is specific for the ~55 kDa TPH protein in Western blots of human dorsal Raphe nucleus. Immunolabeling has been demonstrated in rat brain extracts.
Target Information
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; Tryptophan 5-hydroxylase 1; tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)
Gene Aliases: TPH; TPH1; TPRH; TRPH
UniProt ID: (Human) P17752, (Rabbit) P17290, (Rat) P09810
Entrez Gene ID: (Human) 7166, (Rabbit) 100101558, (Rabbit) 100009100, (Rat) 24848
circadian rhythm
aromatic amino acid family metabolic process
negative regulation of ossification
response to immobilization stress
serotonin biosynthetic process
positive regulation of fat cell differentiation
indolalkylamine biosynthetic process
bone remodeling
oxidation-reduction process
mammary gland alveolus development
serotonin biosynthetic process from tryptophan
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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