Invitrogen

TPH2 Polyclonal Antibody

TPH2 Antibody in Immunoprecipitation (IP)

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TPH2 Antibody (PA1-778) in IP

Immunoprecipitation of TPH2 from raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody (Product # PA1-778). Product # PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody ... View More

TPH2 Antibody in Immunohistochemistry (IHC)

TPH2 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

TPH2 Antibody — Figure 1 Polymerase chain reaction and immunocytochemistry results. (A) Expression of the Cre (left) and TPH2 (right) marker genes. (B) Immunofluorescence in 5-HT-positive neurons in the wild-type group (magnification, x20). (C) 5-HT-positive neurons were lost in the TPH2-knockout group (magnification, x20). Scale bar=20 mum. Mlf, medial longitudinal fasciculus; 5-HT, 5-hydroxytryptamine; TPH2, tryptophan hydroxylase 2. {KO}

Product Details

PA1-778

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:500

Immunoprecipitation (IP)

1:1,000

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2207687

Product Specific Information

PA1-778 detects TPH2 in human, mouse, rat, rabbit and non-human primate samples.

PA1-778 has been successfully used in immunocytochemistry, immunoprecipitation, and Western blot procedures. By Western blot PA1-778 detects a 56 kDa protein representing TPH2.

The PA1-778 immunogen is a synthetic peptide corresponding to the N-terminal region of TPH2.

Target Information

Tryptophan hydroxylase (TPH) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. TPH is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sex-specific modulation of the medial prefrontal cortex by glutamatergic median raphe neurons.

Science advances

Collins SA,Stinson HE,Himes A,Nestor-Kalinoski A,Ninan I

PA1-778 was used in Immunohistochemistry (Frozen) to demonstrate a cell type-specific modulation of the mPFC by MRN (VGLUT3) neurons and reveal a sex-specific role of this neuromodulation in mPFC synaptic plasticity.

Fri Nov 10 00:00:00 UTC 2023

The Mesoscopic Connectome of the Cholinergic Pontomesencephalic Tegmentum.

Frontiers in neuroanatomy

Zhao P,Wang H,Li A,Sun Q,Jiang T,Li X,Gong H

PA1-778 was used in Immunohistochemistry to reveal the integrated cholinergic connectome of the midbrain, thus improving the present understanding of the organizational structure of the pontine-tegmental cholinergic system from its anatomical structure to its functional modulation.

Sat Jul 16 00:00:00 UTC 2022

Depletion of TrkB Receptors From Adult Serotonergic Neurons Increases Brain Serotonin Levels, Enhances Energy Metabolism and Impairs Learning and Memory.

Frontiers in molecular neuroscience

Sahu MP,Pazos-Boubeta Y,Steinzeig A,Kaurinkoski K,Palmisano M,Borowecki O,Piepponen TP,Castrén E

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry (PFA fixed)

Tue May 04 00:00:00 UTC 2021

Sexually dimorphic response of colorectal motility to noxious stimuli in the colorectum in rats.

The Journal of physiology

Horii K,Ehara Y,Shiina T,Naitou K,Nakamori H,Horii Y,Shimaoka H,Saito S,Shimizu Y

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Mon Mar 01 00:00:00 UTC 2021

Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout mice.

Journal of neurochemistry

Haase J,Jones AKC,Mc Veigh CJ,Brown E,Clarke G,Ahnert-Hilger G

PA1-778 was used in Western Blotting to reveal a novel mechanism of SERT regulation and impact our understanding of sex differences in diseases associated with dysregulation of serotonin transmission, such as depression and anxiety.

Fri Oct 01 00:00:00 UTC 2021

Sex and brain region-specific regulation of serotonin transporter activity in synaptosomes in guanine nucleotide-binding protein G(q) alpha knockout mice.

Journal of neurochemistry

Haase J,Jones AKC,Mc Veigh CJ,Brown E,Clarke G,Ahnert-Hilger G

Fri Oct 01 00:00:00 UTC 2021

Serotonergic Plasticity in the Dorsal Raphe Nucleus Characterizes Susceptibility and Resilience to Anhedonia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Prakash N,Stark CJ,Keisler MN,Luo L,Der-Avakian A,Dulcis D

PA1-778 was used in Immunohistochemistry-immunofluorescence to study identifies a novel molecular marker of susceptibility to stress-induced anhedonia, a core symptom of depression, and a means to modulate it.

Wed Jan 15 00:00:00 UTC 2020

Serotonergic Plasticity in the Dorsal Raphe Nucleus Characterizes Susceptibility and Resilience to Anhedonia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Prakash N,Stark CJ,Keisler MN,Luo L,Der-Avakian A,Dulcis D

Wed Jan 15 00:00:00 UTC 2020

Combined exposure to dinotefuran and chronic mild stress counteracts the change of the emotional and monoaminergic neuronal activity induced by either exposure singly despite corticosterone elevation in mice.

The Journal of veterinary medical science

Takada T,Yoneda N,Hirano T,Onaru K,Mantani Y,Yokoyama T,Kitagawa H,Tabuchi Y,Nimako C,Ishizuka M,Ikenaka Y,Hoshi N

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Tue Mar 24 00:00:00 UTC 2020

The Journal of veterinary medical science

Takada T,Yoneda N,Hirano T,Onaru K,Mantani Y,Yokoyama T,Kitagawa H,Tabuchi Y,Nimako C,Ishizuka M,Ikenaka Y,Hoshi N

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Tue Mar 24 00:00:00 UTC 2020

Differential role of dose and environment in initiating and intensifying neurotoxicity caused by MDMA in rats.

BMC pharmacology & toxicology

Shokry IM,Shields CJ,Callanan JJ,Ma Z,Tao R

Published figure using TPH2 polyclonal antibody (Product # PA1-778) in Immunohistochemistry

Mon Aug 05 00:00:00 UTC 2019

Protection of tissue physicochemical properties using polyfunctional crosslinkers.

Nature biotechnology

Park YG,Sohn CH,Chen R,McCue M,Yun DH,Drummond GT,Ku T,Evans NB,Oak HC,Trieu W,Choi H,Jin X,Lilascharoen V,Wang J,Truttmann MC,Qi HW,Ploegh HL,Golub TR,Chen SC,Frosch MP,Kulik HJ,Lim BK,Chung K

PA1-778 was used in Immunohistochemistry-immunofluorescence to demonstrate rapid three-dimensional phenotyping of core needle biopsies and human brain cells.

Mon Dec 17 00:00:00 UTC 2018

Motherhood and infant contact regulate neuroplasticity in the serotonergic midbrain dorsal raphe.

Psychoneuroendocrinology

Holschbach MA,Lonstein JS

PA1778 was used in western blot to assess how plasticity in the midbrain dorsal raphe nucleus affects maternal behavior

Wed Feb 01 00:00:00 UTC 2017

Associations among within-litter differences in early mothering received and later emotional behaviors, mothering, and cortical tryptophan hydroxylase-2 expression in female laboratory rats.

Hormones and behavior

Ragan CM,Harding KM,Lonstein JS

PA1-778 was used in western blot to study how variability in maternal care received among female rat siblings affects the siblings' later emotional and maternal behaviors

Fri Jan 01 00:00:00 UTC 2016

Sympathetic regulation of vascular tone via noradrenaline and serotonin in the rat carotid body as revealed by intracellular calcium imaging.

Brain research

Yokoyama T,Nakamuta N,Kusakabe T,Yamamoto Y

PA1-778 was used in immunohistochemistry - frozen section to study hypoxia-induced chemosensory activity in the carotid body.

Fri Jan 30 00:00:00 UTC 2015

Evaluation of blood-brain barrier permeability in tryptophan hydroxylase 2-knockout mice.

Experimental and therapeutic medicine

Xu CJ,Dai TL,Niu XY,Wang JL,Jin MS

PA1-778 was used in immunohistochemistry to demonstrate that TPH2-knockout mice have a normal blood brain barrier

Sat Nov 01 00:00:00 UTC 2014

Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex.

PloS one

Castro B,Sánchez P,Torres JM,Ortega E

PA1-778 was used in western blot to study the molecular basis for the pathophysiological effects of bisphenol A on the prefrontal cortex of adult rats

Mon May 26 00:00:00 UTC 2014

Differential tissue distribution of tryptophan hydroxylase isoforms 1 and 2 as revealed with monospecific antibodies.

Brain research

Sakowski SA,Geddes TJ,Thomas DM,Levi E,Hatfield JS,Kuhn DM

PA1-778 was used in western blot to investigate the localization of TPH isoforms in various tissues

Wed Apr 26 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: MGC119994; Neuronal tryptophan hydroxylase; TPRH; TRPH; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2; tryptophan hydroxylase 2

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Gene Aliases: ADHD7; AU043594; NTPH; TPH2

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UniProt ID: (Human) Q8IWU9, (Mouse) Q8CGV2, (Rat) Q8CGU9

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Entrez Gene ID: (Rabbit) 100344397, (Human) 121278, (Mouse) 216343, (Rat) 317675

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Function(s)

tryptophan 5-monooxygenase activity iron ion binding amino acid binding monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen metal ion binding molecular_function

Process(es)

circadian rhythm aromatic amino acid family metabolic process response to activity response to nutrient levels serotonin biosynthetic process response to estrogen indolalkylamine biosynthetic process response to glucocorticoid response to calcium ion oxidation-reduction process cellular response to lithium ion serotonin biosynthetic process from tryptophan metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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TPH2 Polyclonal Antibody

TPH2 Antibody (PA1-778) in IP

Product Details

PA1-778

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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