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TPM1 Antibody (MA5-32982) in WB
Western blot analysis of Tropomyosin(36/39 kDa) in, Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked ... View More
Product Details
MA5-32982
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
TM31
Immunogen
Chicken gizzard tropomyosin.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
ascites/1.2% sodium acetate with 2mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2802616
Product Specific Information
Reconstitute by adding 1 mL of PBS buffer, yielding a concentration of 100 µg/mL.
Positive Control - WB: rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha tropomyosin; Alpha-tropomyosin; alpha-tropomyosin 1; alpha-tropomyosin 2; alpha-tropomyosin of skeletal fast muscle; cardiomyopathy, hypertrophic 3; epididymis secretory protein Li 265; hepatoma alpha tropomyosin; sarcomeric tropomyosin kappa; smooth muscle alpha-tropomyosin; striated muscle alpha-tropomyosin; TPM1-alpha; tropomyosin; tropomyosin (CTm4); tropomyosin (CTm7); tropomyosin 3 alpha; Tropomyosin alpha-1 chain; Tropomyosin-1
Gene Aliases: AA986836; AI854628; alpha-fTM; Alpha-tm; C15orf13; CMD1Y; CMH3; HEL-S-265; HTM-alpha; LVNC9; TM2; Tm3; Tma2; Tmpa; TMSA; Tpm-1; TPM1; Tpma
UniProt ID: (Human) P09493, (Chicken) P04268, (Rat) P04692, (Mouse) P58771
Entrez Gene ID: (Human) 7168, (Chicken) 396366, (Rat) 24851, (Mouse) 22003
in utero embryonic development
positive regulation of heart rate by epinephrine
movement of cell or subcellular component
muscle contraction
regulation of muscle contraction
cytoskeleton organization
regulation of heart contraction
regulation of cell shape
muscle filament sliding
negative regulation of cell migration
ruffle organization
positive regulation of ATPase activity
cellular response to reactive oxygen species
wound healing
sarcomere organization
positive regulation of cell adhesion
positive regulation of stress fiber assembly
ventricular cardiac muscle tissue morphogenesis
cardiac muscle contraction
negative regulation of vascular smooth muscle cell proliferation
negative regulation of vascular associated smooth muscle cell migration
regulation of ATPase activity
actin filament capping
embryo development
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