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TPM4 Antibody (720311) in ICC/IF
For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous Tropomyosin-4 using Anti- TPM-4 Rabbit Polyclonal Antibody (Product # 720311, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TPM-4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). ... View More
Product Details
720311
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human TPM4 (aa 21-34, 229-244)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 30% glycerol
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633255
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Pig, Horse and Rat
Target Information
TPM4 (tropomyosin 4) is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. TPM4 plays a central role in association with the troponin complex in the calcium dependent regulations of vertebrate striated muscle contraction. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 108; TM30p1; tropomyosin; Tropomyosin alpha-4 chain; Tropomyosin-4
Gene Aliases: 2610528G24Rik; HEL-S-108; TPM4; Tpm4.2; Tpm4.2cy
UniProt ID: (Human) P67936, (Mouse) Q6IRU2
Entrez Gene ID: (Human) 7171, (Mouse) 326618
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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